Mycobacterium phage Koella
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Detailed Information for Phage Koella
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByKoita Fofana and Gabrielle Findley
Year Found2015
Location FoundNyack, NY USA
Finding InstitutionNyack College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates41.092736 N, 73.929103 W Map
Discovery NotesSoil was dark brown, gritty top soil. It collected around shrubs and grass.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 5, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage253
Genome length (bp)54118
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGACGGCGC
GC Content61.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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AbbyNormal (None)

Grib (None)

Kishmeesh (None)

KitCat (None)

MightyMorph (None)

Puck (None)

Renaud18 (None)

WIVsmall1220 (None)

Alexphander (F1)

Anaxagorus (F1)

Ant1Dodson (F1)

ArcusAngelus (F1)

Ardmore (F1)

Arienne (F1)

Asmart (F1)

Awesomesauce (F1)

BabytheFifth (F1)

Barson (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

BoomCrackaPop (F1)

Boomer (F1)

Braemar (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

Cakepop (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chhibber (F1)

Chikondi (F1)

Chuckly (F1)

Clifton (F1)

Clover (F1)

CoolGuy (F1)

Cornucopia (F1)

Daenerys (F1)

Danda (F1)

Dante (F1)

DaWorst (F1)

DCrust15 (F1)

DeadP (F1)

Desmond (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Docouto (F1)

Dorothy (F1)

DotProduct (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Estave1 (F1)

Estella (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Freddie4 (F1)

Fruitloop (F1)

FunOne (F1)

Galactic (F1)

Gardregs (F1)

Gendisarray (F1)

Genesis (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GreatPumpkin (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Judylove (F1)

Kalikimaka (F1)

Katya (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

Latretium (F1)

LillyLatzon (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Lysinator (F1)

MacDrage (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

McDiddy (F1)

Melissauren88 (F1)

MerrillCO18 (F1)

Mille (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Mozy (F1)

Ms6 (F1)

Mutaforma13 (F1)

Myosotis (F1)

Nemain (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

Noodle (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Peanut21s (F1)

Phabio (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

PhrankthaTank (F1)

Piper2020 (F1)

Pippy (F1)

Plackberry (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

Prometheus (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

Rocktrap (F1)

RockyHorror (F1)

Rombolo (F1)

Royals2015 (F1)

Ruby (F1)

S2Kari (F1)

S2TSquared (F1)

Saal (F1)

SaltyMike (F1)

Sandalphon (F1)

SassyB (F1)

Scottish (F1)

Seagreen (F1)

SG4 (F1)

Sharpshooter (F1)

Shauna1 (F1)

SherL (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

Taus (F1)

TDanisky (F1)

TonaldTDrump (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Wachhund (F1)

Wee (F1)

WheatBerry18 (F1)

Whouxphf (F1)

WillSterrel (F1)

WIVsmall (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotation StatusIn GenBank
Plaque NotesPlaque Morphology (after 24hrs):
Diameter: 1.5mm
Clarity of Plaque: Clear
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Koella_1
(terminase small subunit)

Koella_2
(terminase large subunit)

Koella_3
(portal protein)

Koella_4
(capsid maturation protease)

Koella_5
(scaffolding protein)

Koella_6
(major capsid protein)

Koella_7
(head-to-tail connector complex protein)

Koella_8
(head-to-tail connector complex protein)

Koella_9
(head-to-tail connector complex protein)

Koella_10
(head-to-tail connector complex protein)

Koella_11
(major tail protein)

Koella_12
(tail assembly chaperone)

Koella_13
(tail assembly chaperone)

Koella_14
(tape measure protein)

Koella_15
(minor tail protein)

Koella_16
(minor tail protein)

Koella_17
(minor tail protein)

Koella_18
(minor tail protein)

Koella_19
(minor tail protein)

Koella_20

Koella_21

Koella_22
(membrane protein)

Koella_23

Koella_24
(lysin A)

Koella_25
(lysin B)

Koella_26
(holin)

Koella_27
(membrane protein)

Koella_28

Koella_29
(DNA polymerase III subunit)

Koella_30

Koella_31
(membrane protein)

Koella_32

Koella_33
(membrane protein)

Koella_34
(DNA binding protein)

Koella_35

Koella_36
(transposase)

Koella_38
(integrase)

Koella_39
(membrane protein)

Koella_40
(immunity repressor)

Koella_41
(Cro protein)

Koella_42

Koella_43

Koella_44
(excise)

Koella_45

Koella_46

Koella_47

Koella_48

Koella_49

Koella_50
(membrane protein)

Koella_51
(WhiB family transcription factor)

Koella_52
(helix-turn-helix DNA binding domain protein)

Koella_53
(WhiB family transcription factor)

Koella_54
(helix-turn-helix DNA binding domain protein)

Koella_55

Koella_56

Koella_57

Koella_58
(DNA recombinase)

Koella_59
(HNH endonuclease)

Koella_60
(DNA helicase)

Koella_61
(DNA methylase)

Koella_62
(membrane protein)

Koella_63

Koella_64

Koella_65
(G-I-Y Y-I-G endonuclease)

Koella_66
(helix-turn-helix DNA binding domain protein)

Koella_67

Koella_68

Koella_69

Koella_70

Koella_71
(MPME1 protein)

Koella_72

Koella_73

Koella_74

Koella_75

Koella_76

Koella_77

Koella_78

Koella_79

Koella_80

Koella_81

Koella_82

Koella_83

Koella_84
(G-I-Y Y-I-G endonuclease)

Koella_85

Koella_86

Koella_87
(membrane protein)

Koella_88

Koella_89

Koella_90

Koella_91

Koella_92

Koella_93

Koella_94
(glycosyltransferase)

Koella_95

Koella_96
(glycosyltransferase)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH316564
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 3.4 x 10^10 pfu/mL
Date of SEA Lysate Titering Nov 2, 2015
Pitt Freezer Box# 22
Pitt Freezer Box Grid# G6
Available Files
GenBank File for PhameratorDownload