The Actinobacteriophage Database

Mycobacterium phage Phasih

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Detailed Information for Phage Phasih
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Former names	Mufasih
Found By	James Ramirez
Year Found	2015
Location Found	Los Angeles, CA USA
Finding Institution	University of California, Los Angeles
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	35°C
GPS Coordinates	34.0673 N, 118.4526 W Map
Discovery Notes	Sample collected by James Ramirez. Depth of sample: 5cm Sample-site descriptors: Potted Plant in Midvale Plaza, Westwood, soil was moist Temperature: 70°F Phage isolated, purified, characterized, and genome annotated by Teams PWA and PITYMI. Team Phagez wit Attitudez (PWA): David Chernobylsky Galen Law James Ramirez Julien Trevare Phage It Til You Make It (PITYMI): Sean Laiola Kathy Ordas Jahzeel Paguntalan Anna Shvartsur TA: Fasih Ahsan Instructor: Giorgia Pirino Laboratory Support: Krisanavane Reddi
Naming Notes	Combination of Mufasah from the Lion King and Fasih, the section Teaching Assistant.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 4, 2016
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	354
Genome length (bp)	56125
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CGGAAGGCGC
GC Content	61.1%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	F
Subcluster	F1
Cluster Life Cycle	Temperate
Lysogeny Notes	Plaques are very clear, but integrase gene is present.
Other Cluster Members	Click to View MulchSalad (None) Soul22 (None) Akhila (F1) Alexphander (F1) AlpineSix (F1) ArcusAngelus (F1) Ardmore (F1) Aubs (F1) Awesomesauce (F1) Batiatus (F1) Beakin (F1) BigPhil (F1) Bipolar (F1) Blexus (F1) BlueCrab (F1) BobaPhett (F1) Bobi (F1) BodEinwohner17 (F1) Boomer (F1) Brocalys (F1) Bubbles123 (F1) Burwell21 (F1) BuzzLyseyear (F1) ByChance (F1) Byougenkin (F1) Cabrinians (F1) CaptainTrips (F1) Cerasum (F1) Che8 (F1) ChickenDinner (F1) Chuckly (F1) Clifton (F1) Coco12 (F1) Cornucopia (F1) DaddyRickover (F1) Daenerys (F1) Dante (F1) DaWorst (F1) DeadP (F1) DillTech15 (F1) DirtMcgirt (F1) DLane (F1) Donkeykong (F1) Doomphist (F1) Dorothy (F1) DotProduct (F1) Doug (F1) Drago (F1) DRBy19 (F1) Eish (F1) EleanorGeorge (F1) Emma (F1) Empress (F1) Enby (F1) Eradicator (F1) Estave1 (F1) Fancypants (F1) Filuzino (F1) Firehouse51 (F1) Flathead (F1) Florinda (F1) Frankie (F1) Fruitloop (F1) Galactic (F1) Gandalph (F1) Geralt (F1) GigiOuiOui (F1) Girafales (F1) Girr (F1) GUmbie (F1) Hades (F1) Hamulus (F1) Harley (F1) Hegedechwinu (F1) Hlubikazi (F1) Ibhubesi (F1) Inventum (F1) IrishSherpFalk (F1) JalFarm20 (F1) James (F1) Jarcob (F1) Job42 (F1) JoeyJr (F1) Juice456 (F1) Karhdo (F1) Kenuha5 (F1) Kersh (F1) Kimberlium (F1) KingMidas (F1) Kingsley (F1) Koella (F1) Krakatau (F1) KristaRAM (F1) LastJedi (F1) Latretium (F1) Leozinho (F1) Leperchaun (F1) LilMoolah (F1) Lizziana (F1) Llama (F1) Llij (F1) Lorde (F1) LunaBlu (F1) Madiba (F1) MadMen (F1) Mahavrat (F1) Mandlovu (F1) Mantra (F1) Maravista (F1) Martik (F1) Mattes (F1) Melissauren88 (F1) MilanaBonita (F1) MilleniumForce (F1) MinionDave (F1) Minnie (F1) Misha28 (F1) MisterCuddles (F1) Modragons (F1) Moonbeam (F1) Mova (F1) Mozy (F1) Ms6 (F1) Mutaforma13 (F1) Nimbo (F1) Nitzel (F1) Nivrat (F1) NormanBulbieJr (F1) Ochi17 (F1) OfUltron (F1) Ogopogo (F1) Oksu (F1) OldBen (F1) OlympiaSaint (F1) Ovechkin (F1) OwlsT2W (F1) Pacc40 (F1) Phalconet (F1) Phanphagia (F1) PHappiness (F1) Phasih (F1) Phatniss (F1) Piper2020 (F1) Pippy (F1) Plumbus (F1) PMC (F1) Poenanya (F1) Polka14 (F1) Pollywog (F1) PopTart (F1) Priscilla (F1) QuickMath (F1) Quico (F1) Radiance (F1) Ramsey (F1) RedBird (F1) Rialto (F1) Rita (F1) RitaG (F1) Rockne (F1) RockyHorror (F1) Royals2015 (F1) Ruby (F1) Saal (F1) Sabbb (F1) Sandalphon (F1) Sarma624 (F1) Sassafras (F1) SassyB (F1) Scottish (F1) Seabastian (F1) Seagreen (F1) SG4 (F1) Shauna1 (F1) ShiLan (F1) ShroomBoi (F1) SimranZ1 (F1) SiSi (F1) Sparkdehlily (F1) Spartacus (F1) Spikelee (F1) Spoonbill (F1) StAnnes (F1) Stap (F1) Starcevich (F1) Strokeseat (F1) SuperGrey (F1) SwagPigglett (F1) Taj (F1) TDanisky (F1) TootsiePop (F1) Tootsieroll (F1) Tweety (F1) UncleRicky (F1) Velveteen (F1) Veteran (F1) VRedHorse (F1) Wachhund (F1) Wee (F1) Whatsapiecost (F1) Whouxphf (F1) WillSterrel (F1) XFactor (F1) Yorick (F1) Zerg (F1) Zizzle (F1) Avani (F2) Che9d (F2) Demsculpinboyz (F2) Jabbawokkie (F2) Yoshi (F2) Zapner (F2) Squirty (F3) Renaud18 (F4) TChen (F4) ThetaBob (F4) Cornie (F5)
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	Clear plaques Average plaque size: 2.74mm
Morphotype	Siphoviridae
Number of Genes	99
Has been Phamerated?	Yes
Gene List	Click to View Phasih_1 (terminase small subunit) Phasih_2 (terminase large subunit) Phasih_3 (portal protein) Phasih_4 (capsid maturation protease) Phasih_5 (scaffolding protein) Phasih_6 (major capsid protein) Phasih_7 (head-to-tail adaptor) Phasih_8 (head-to-tail stopper) Phasih_9 Phasih_10 (tail terminator) Phasih_11 (major tail protein) Phasih_12 (tail assembly chaperone) Phasih_13 (tail assembly chaperone) Phasih_14 (HNH endonuclease) Phasih_15 (tape measure protein) Phasih_16 (minor tail protein) Phasih_17 (minor tail protein) Phasih_18 (minor tail protein) Phasih_19 (minor tail protein) Phasih_20 (minor tail protein) Phasih_21 (minor tail protein) Phasih_22 (minor tail protein) Phasih_23 Phasih_24 (minor tail protein) Phasih_25 (minor tail protein) Phasih_26 (helix-turn-helix DNA binding domain protein) Phasih_27 Phasih_28 Phasih_29 Phasih_30 Phasih_31 (lysin A) Phasih_32 (lysin B) Phasih_33 (holin) Phasih_34 Phasih_35 Phasih_36 (DNA polymerase III subunit) Phasih_37 Phasih_38 Phasih_39 Phasih_40 Phasih_41 (integrase) Phasih_42 Phasih_43 (immunity repressor) Phasih_44 (CRO protein) Phasih_45 (antirepressor) Phasih_46 Phasih_47 Phasih_48 Phasih_49 Phasih_50 Phasih_51 Phasih_52 Phasih_53 Phasih_54 Phasih_55 (WhiB family transcription factor) Phasih_56 Phasih_57 (WhiB family transcription factor) Phasih_58 (helix-turn-helix DNA binding domain protein) Phasih_59 Phasih_60 Phasih_61 Phasih_62 (AAA-ATPase) Phasih_63 Phasih_64 Phasih_65 (DNA methylase) Phasih_66 Phasih_67 Phasih_68 Phasih_69 Phasih_70 Phasih_71 Phasih_72 Phasih_73 Phasih_74 Phasih_75 Phasih_76 Phasih_77 Phasih_78 Phasih_79 Phasih_80 Phasih_81 Phasih_82 Phasih_83 Phasih_84 Phasih_85 Phasih_86 Phasih_87 Phasih_88 Phasih_89 Phasih_90 Phasih_91 Phasih_92 Phasih_93 Phasih_94 Phasih_95 Phasih_96 Phasih_97 (glycosyltransferase) Phasih_98 Phasih_99 (glycosyltransferase) Phasih_100 (HNH endonuclease)
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MF919526
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	23
Pitt Freezer Box Grid#	E8
Available Files
Plaque Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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