Detailed Information for Phage Phasih |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Former names | Mufasih |
Found By | James Ramirez |
Year Found | 2015 |
Location Found | Los Angeles, CA USA |
Finding Institution | University of California, Los Angeles |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 35°C |
GPS Coordinates | 34.0673 N, 118.4526 W Map |
Discovery Notes | Sample collected by James Ramirez. Depth of sample: 5cm Sample-site descriptors: Potted Plant in Midvale Plaza, Westwood, soil was moist Temperature: 70°F
Phage isolated, purified, characterized, and genome annotated by Teams PWA and PITYMI.
Team Phagez wit Attitudez (PWA): David Chernobylsky Galen Law James Ramirez Julien Trevare
Phage It Til You Make It (PITYMI): Sean Laiola Kathy Ordas Jahzeel Paguntalan Anna Shvartsur
TA: Fasih Ahsan Instructor: Giorgia Pirino Laboratory Support: Krisanavane Reddi |
Naming Notes | Combination of Mufasah from the Lion King and Fasih, the section Teaching Assistant. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 4, 2016 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 354 |
Genome length (bp) | 56125 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CGGAAGGCGC |
GC Content | 61.1% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | F |
Subcluster | F1 |
Cluster Life Cycle | Temperate |
Lysogeny Notes | Plaques are very clear, but integrase gene is present. |
Other Cluster Members |
|
Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | Clear plaques Average plaque size: 2.74mm
|
Morphotype | Siphoviridae |
Number of Genes | 99 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MF919526 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
23 |
Pitt Freezer Box Grid# |
E8 |
Available Files |
Plaque Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |