| Detailed Information for Phage Phasih |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Mufasih |
| Found By | James Ramirez |
| Year Found | 2015 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 35°C |
| GPS Coordinates | 34.0673 N, 118.4526 W Map |
| Discovery Notes | Sample collected by James Ramirez. Depth of sample: 5cm
Sample-site descriptors: Potted Plant in Midvale Plaza, Westwood, soil was moist
Temperature: 70°F
Phage isolated, purified, characterized, and genome annotated by Teams PWA and PITYMI.
Team Phagez wit Attitudez (PWA):
David Chernobylsky
Galen Law
James Ramirez
Julien Trevare
Phage It Til You Make It (PITYMI):
Sean Laiola
Kathy Ordas
Jahzeel Paguntalan
Anna Shvartsur
TA: Fasih Ahsan
Instructor: Giorgia Pirino
Laboratory Support: Krisanavane Reddi |
| Naming Notes | Combination of Mufasah from the Lion King and Fasih, the section Teaching Assistant. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 4, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 354 |
| Genome length (bp) | 56125 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGAAGGCGC |
| GC Content | 61.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Plaques are very clear, but integrase gene is present. |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Clear plaques
Average plaque size: 2.74mm
|
| Morphotype | Siphoviridae |
| Number of Genes | 99 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF919526 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
23 |
| Pitt Freezer Box Grid# |
E8 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
