The Actinobacteriophage Database

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Detailed Information for Phage FreddyB
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Diamond Love, Matilda McDaniel, Sherlyn Diaz
Year Found	2023
Location Found	Longview, TX United States
Finding Institution	LeTourneau University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	37°C
GPS Coordinates	32.463333 N, 94.7275 W Map
Discovery Notes	At 9:52 CDT on August 22, 2023, the soil sample was collected from around the pond behind the Glaske Center for Engineering, Science, and Technology at LeTourneau University. After removing the top foliage, a hole was dug, and it was approximately 11 cm wide and 4 cm deep. The soil was dark, moist, rich, and contained lots of roots and worms. The weather was sunny, with a clear sky and an ambient temperature of 26 °C.
Naming Notes	Because we were so thankful for our teacher, Dr. Frederick Baliraine, helping us with the lab after we had failed the first time, we decided to name our phage after him as a way of acknowledging him for facilitating our success.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Oct 30, 2023
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina MiSeq
Read Type	Single-end reads
Read Length	150 bp
Approximate Shotgun Coverage	1175
Genome length (bp)	58318
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CGGACGGCGC
GC Content	61.7%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	F
Subcluster	F1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Blarby (None) MulchSalad (None) Akhila (F1) Alexphander (F1) Aloeri (F1) AlpineSix (F1) ArcusAngelus (F1) Ardmore (F1) Aubs (F1) Awesomesauce (F1) Batiatus (F1) Beakin (F1) BigPhil (F1) Bipolar (F1) Blexus (F1) BlueCrab (F1) BobaPhett (F1) Bobi (F1) BodEinwohner17 (F1) Boomer (F1) Brocalys (F1) Brookers (F1) Brushbloom (F1) Bubbles123 (F1) Burwell21 (F1) BuzzLyseyear (F1) ByChance (F1) Byougenkin (F1) Cabrinians (F1) CaptainTrips (F1) Cerasum (F1) Che8 (F1) Cheesepuff (F1) Chevrolet (F1) ChickenDinner (F1) Chuckly (F1) Clifton (F1) Coco12 (F1) Corium (F1) Cornucopia (F1) Crumble (F1) DaddyRickover (F1) Daenerys (F1) Dante (F1) DaWorst (F1) DeadP (F1) Deb65 (F1) DillTech15 (F1) DirtMcgirt (F1) DLane (F1) DocMcStuffins (F1) Donkeykong (F1) Doomphist (F1) DoRead (F1) Dorothy (F1) DosHalletts (F1) DotProduct (F1) Doug (F1) Drago (F1) DRBy19 (F1) Eish (F1) EleanorGeorge (F1) EmiMonkey (F1) Emma (F1) Emmaloid (F1) Empress (F1) Enby (F1) Eradicator (F1) Estave1 (F1) Fancypants (F1) Fastidio (F1) Filuzino (F1) Firehouse51 (F1) Flathead (F1) Florinda (F1) Frankie (F1) FreddyB (F1) Fruitloop (F1) Galactic (F1) Gandalph (F1) Geralt (F1) GigiOuiOui (F1) Girafales (F1) Girr (F1) Gorge (F1) Grimmer (F1) GUmbie (F1) Guppsters (F1) Hades (F1) Hamulus (F1) Harley (F1) Hegedechwinu (F1) Hlubikazi (F1) Ibhubesi (F1) IbOuu (F1) IMpilo (F1) Inventum (F1) IrishSherpFalk (F1) JalFarm20 (F1) James (F1) Jant (F1) Jarcob (F1) Jinglebell (F1) Job42 (F1) JoeyJr (F1) Juice456 (F1) Juniper1 (F1) Karhdo (F1) Kenuha5 (F1) Kersh (F1) Kimberlium (F1) KingJulian (F1) KingMidas (F1) Kingsley (F1) Koella (F1) Krakatau (F1) KristaRAM (F1) LastJedi (F1) Latretium (F1) Leozinho (F1) Leperchaun (F1) LilMoolah (F1) LittleShirley (F1) Lizziana (F1) Llama (F1) Llij (F1) Lorde (F1) LunaBlu (F1) Madiba (F1) MadMen (F1) Mahavrat (F1) Mandlovu (F1) Mantra (F1) Maravista (F1) Marker (F1) Martik (F1) Mattes (F1) Melissauren88 (F1) MilanaBonita (F1) MilleniumForce (F1) MinionDave (F1) Minnie (F1) Misha28 (F1) MisterCuddles (F1) Modragons (F1) Moonbeam (F1) Mova (F1) Mozy (F1) Ms6 (F1) MulchExplorer (F1) Mutaforma13 (F1) NewHope4 (F1) Nimbo (F1) Nitzel (F1) Nivrat (F1) NormanBulbieJr (F1) Ochi17 (F1) OfUltron (F1) Ogopogo (F1) Oksu (F1) OldBen (F1) OlympiaSaint (F1) Ovechkin (F1) OwlsT2W (F1) Pacc40 (F1) Phalconet (F1) Phanphagia (F1) PHappiness (F1) Phasih (F1) Phatniss (F1) PhesterPhotato (F1) Philus (F1) PhlipPhlop (F1) Piper2020 (F1) Pippy (F1) Plumbus (F1) PMC (F1) Poenanya (F1) Polka14 (F1) Pollywog (F1) Polo2Bam (F1) PopTart (F1) Priscilla (F1) QuickMath (F1) Quico (F1) Radiance (F1) Ramsey (F1) RedBird (F1) Rialto (F1) Rita (F1) RitaG (F1) Rockne (F1) RockyHorror (F1) Royals2015 (F1) Ruby (F1) Saal (F1) Sabbb (F1) Sandalphon (F1) Sarma624 (F1) Sassafras (F1) SassyB (F1) Scottish (F1) Seabastian (F1) Seagreen (F1) SG4 (F1) Shauna1 (F1) ShiLan (F1) ShowerHandel (F1) ShroomBoi (F1) SimranZ1 (F1) SiSi (F1) Slim (F1) Sparkdehlily (F1) Spartacus (F1) Spikelee (F1) Spoonbill (F1) StAnnes (F1) Stap (F1) Starcevich (F1) Strokeseat (F1) SuperGrey (F1) SwagPigglett (F1) Taj (F1) Tchotchke (F1) TDanisky (F1) TootsiePop (F1) Tootsieroll (F1) Totinger (F1) Tweety (F1) UncleRicky (F1) Velveteen (F1) Veteran (F1) Violac (F1) Vivum (F1) VRedHorse (F1) Wachhund (F1) Wee (F1) Whatsapiecost (F1) Whouxphf (F1) WillSterrel (F1) XFactor (F1) Yorick (F1) Zerg (F1) Zizzle (F1) Avani (F2) Che9d (F2) Demsculpinboyz (F2) Jabbawokkie (F2) SilverChicken (F2) Soul22 (F2) Yoshi (F2) Zapner (F2) Squirty (F3) LunaStella (F4) Renaud18 (F4) TChen (F4) ThetaBob (F4) Cornie (F5) Toron (F6)
Annotating Institution	LeTourneau University
Annotation Status	In GenBank
Plaque Notes	FreddyB, produced small, cloudy plaques. They were approximately 1.13 mm in diameter (range 0.7-2.2 mm; n = 10).
Morphotype	Siphoviridae
Number of Genes	113
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View FreddyB_1 (terminase small subunit) FreddyB_2 (terminase large subunit) FreddyB_3 (portal protein) FreddyB_4 (capsid maturation protease) FreddyB_5 (scaffolding protein) FreddyB_6 (major capsid protein) FreddyB_7 (head-to-tail adaptor) FreddyB_8 (head-to-tail stopper) FreddyB_9 FreddyB_10 (tail terminator) FreddyB_11 (major tail protein) FreddyB_12 (tail assembly chaperone) FreddyB_13 (tail assembly chaperone) FreddyB_14 (tape measure protein) FreddyB_15 (minor tail protein) FreddyB_16 (minor tail protein) FreddyB_17 (minor tail protein) FreddyB_18 (minor tail protein) FreddyB_19 (minor tail protein) FreddyB_20 (minor tail protein) FreddyB_21 FreddyB_22 FreddyB_23 FreddyB_24 FreddyB_25 (helix-turn-helix DNA binding domain protein) FreddyB_26 FreddyB_27 FreddyB_28 FreddyB_29 FreddyB_30 (lysin A) FreddyB_31 (lysin B) FreddyB_32 (holin) FreddyB_33 FreddyB_34 FreddyB_35 (DnaQ-like DNA polymerase III subunit) FreddyB_36 FreddyB_37 FreddyB_38 FreddyB_39 FreddyB_40 FreddyB_41 FreddyB_42 FreddyB_43 (tyrosine integrase) FreddyB_44 FreddyB_45 FreddyB_46 (immunity repressor) FreddyB_47 (Cro protein) FreddyB_48 FreddyB_49 FreddyB_50 (excise protein) FreddyB_51 FreddyB_52 FreddyB_53 FreddyB_54 FreddyB_55 FreddyB_56 FreddyB_57 FreddyB_58 (WhiB family transcription factor) FreddyB_59 (helix-turn-helix DNA binding domain protein) FreddyB_60 (WhiB family transcription factor) FreddyB_61 (helix-turn-helix DNA binding domain protein) FreddyB_62 FreddyB_63 FreddyB_64 FreddyB_65 (RecA-like DNA recombinase) FreddyB_66 (HNH endonuclease) FreddyB_67 (DNA methyltransferase) FreddyB_68 FreddyB_69 (DNA methyltransferase) FreddyB_70 FreddyB_71 FreddyB_72 FreddyB_73 (DNA methyltransferase) FreddyB_74 (ribbon-helix-helix DNA binding domain protein) FreddyB_75 FreddyB_76 FreddyB_77 FreddyB_78 FreddyB_79 FreddyB_80 FreddyB_81 FreddyB_82 (RepA-like replication initiator) FreddyB_83 (helicase loader) FreddyB_84 FreddyB_85 FreddyB_86 FreddyB_87 FreddyB_88 FreddyB_89 FreddyB_90 FreddyB_91 (MPME 1 protein) FreddyB_92 FreddyB_93 FreddyB_94 FreddyB_95 FreddyB_96 FreddyB_97 FreddyB_98 FreddyB_99 FreddyB_100 FreddyB_101 (G-I-Y Y-I-G endonuclease) FreddyB_102 FreddyB_103 FreddyB_104 FreddyB_105 FreddyB_106 FreddyB_107 FreddyB_108 FreddyB_109 FreddyB_110 (glycosyltransferase) FreddyB_111 FreddyB_112 (glycosyltransferase) FreddyB_113 (HNH endonuclease)
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	PP537960
Refseq Number	None yet
Archiving Info
Archiving status	Archived
SEA Lysate Titer	3.2 x 10^10 PFU/ml
Pitt Freezer Box#	168
Pitt Freezer Box Grid#	B3
Available Files
GenBank File for Phamerator	Download

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