Mycobacterium phage Stap
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Detailed Information for Phage Stap
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByPhilip Kerber
Year Found2022
Location FoundSouth Haven, MI United States
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature35°C
GPS Coordinates42.35426 N, 86.28723 W Map
Discovery NotesSoil sample was collected on 9/3/22 @ 5:55 PM. The soil was damp and spongy and was collected approximately 3 inches down into a rotting tree stump in a nature preserve. The air temperature at the time was 79°F.
Naming NotesThis phage is named after my high school chemistry teacher. His enthusiasm for science still inspires me every day.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 13, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage868
Genome length (bp)54395
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCCGATGGCAT
GC Content61.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Blarby (None)

MulchSalad (None)

Akhila (F1)

Alexphander (F1)

AlpineSix (F1)

ArcusAngelus (F1)

Ardmore (F1)

Aubs (F1)

Awesomesauce (F1)

Batiatus (F1)

Beakin (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BlueCrab (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chevrolet (F1)

ChickenDinner (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Crumble (F1)

DaddyRickover (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

Deb65 (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

DocMcStuffins (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DosHalletts (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Eradicator (F1)

Estave1 (F1)

Fancypants (F1)

Fastidio (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

FreddyB (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

GigiOuiOui (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Guppsters (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

IbOuu (F1)

IMpilo (F1)

Inventum (F1)

IrishSherpFalk (F1)

JalFarm20 (F1)

James (F1)

Jarcob (F1)

Jinglebell (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Juniper1 (F1)

Karhdo (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

Leozinho (F1)

Leperchaun (F1)

LilMoolah (F1)

LittleShirley (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

LunaBlu (F1)

Madiba (F1)

MadMen (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Maravista (F1)

Marker (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilanaBonita (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Ms6 (F1)

MulchExplorer (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

Oksu (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phalconet (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

PhesterPhotato (F1)

Philus (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Radiance (F1)

Ramsey (F1)

RedBird (F1)

Rialto (F1)

Rita (F1)

RitaG (F1)

Rockne (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sabbb (F1)

Sandalphon (F1)

Sarma624 (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

ShowerHandel (F1)

ShroomBoi (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Stap (F1)

Starcevich (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tootsieroll (F1)

Totinger (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

VRedHorse (F1)

Wachhund (F1)

Wee (F1)

Whatsapiecost (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Yorick (F1)

Zerg (F1)

Zizzle (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Soul22 (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

LunaStella (F4)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Toron (F6)

Annotating InstitutionHope College
Annotation StatusIn GenBank
Plaque NotesThe initial morphology after 24 hours of incubation is a clear plaque about 1 mm in size. However, after a week in storage at 4°C, the plaque will gain a crescent comet tail about 3mm in diameter.
Has been Phamerated?Yes
Gene List

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Stap_1
(HNH endonuclease)

Stap_2
(terminase small subunit)

Stap_3
(terminase large subunit)

Stap_4
(portal protein)

Stap_5
(capsid maturation protease)

Stap_6
(scaffolding protein)

Stap_7
(major capsid protein)

Stap_8
(head-to-tail adaptor)

Stap_9
(head-to-tail stopper)

Stap_10

Stap_11
(tail terminator)

Stap_12
(major tail protein)

Stap_13
(tail assembly chaperone)

Stap_14
(tail assembly chaperone)

Stap_15
(tape measure protein)

Stap_16
(minor tail protein)

Stap_17
(minor tail protein)

Stap_18
(minor tail protein)

Stap_19
(minor tail protein)

Stap_20
(minor tail protein)

Stap_21
(minor tail protein)

Stap_22

Stap_23

Stap_24

Stap_25

Stap_26
(helix-turn-helix DNA binding domain protein)

Stap_27

Stap_28

Stap_29

Stap_30

Stap_31
(lysin A)

Stap_32
(lysin B)

Stap_33
(holin)

Stap_34

Stap_35

Stap_36
(DnaQ-like DNA polymerase III subunit)

Stap_37

Stap_38

Stap_39

Stap_40

Stap_41

Stap_42
(tyrosine integrase)

Stap_43

Stap_44
(immunity repressor)

Stap_45
(cro protein)

Stap_46
(antirepressor)

Stap_47

Stap_48

Stap_49
(excise)

Stap_50

Stap_51

Stap_52

Stap_53

Stap_54

Stap_55
(WhiB family transcription factor)

Stap_56

Stap_57
(WhiB family transcription factor)

Stap_58
(helix-turn-helix DNA binding domain protein)

Stap_59

Stap_60

Stap_61
(RecA-like DNA recombinase)

Stap_62

Stap_63
(HNH endonuclease)

Stap_64

Stap_65

Stap_66

Stap_67

Stap_68
(RepA-like replication initiator)

Stap_69

Stap_70

Stap_71

Stap_72

Stap_73

Stap_74

Stap_75

Stap_76

Stap_77

Stap_78

Stap_79

Stap_80

Stap_81

Stap_82

Stap_83

Stap_84

Stap_85

Stap_86

Stap_87

Stap_88

Stap_89
(helix-turn-helix DNA binding domain protein)

Stap_90

Stap_91

Stap_92

Stap_93

Stap_94

Stap_95

Stap_96
(glycosyltransferase)

Stap_97
(kinase)

Stap_98

Stap_99
(glycosyltransferase)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOR475252
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 151
Pitt Freezer Box Grid# H2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload