| Detailed Information for Phage Stap |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Philip Kerber |
| Year Found | 2022 |
| Location Found | South Haven, MI United States |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 35°C |
| GPS Coordinates | 42.35426 N, 86.28723 W Map |
| Discovery Notes | Soil sample was collected on 9/3/22 @ 5:55 PM. The soil was damp and spongy and was collected approximately 3 inches down into a rotting tree stump in a nature preserve. The air temperature at the time was 79°F. |
| Naming Notes | This phage is named after my high school chemistry teacher. His enthusiasm for science still inspires me every day. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 13, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 868 |
| Genome length (bp) | 54395 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CCGATGGCAT |
| GC Content | 61.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Hope College |
| Annotation Status | In GenBank |
| Plaque Notes | The initial morphology after 24 hours of incubation is a clear plaque about 1 mm in size. However, after a week in storage at 4°C, the plaque will gain a crescent comet tail about 3mm in diameter. |
| Number of Genes | 99 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR475252 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
151 |
| Pitt Freezer Box Grid# |
H2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
