Detailed Information for Phage Stap |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Philip Kerber |
Year Found | 2022 |
Location Found | South Haven, MI United States |
Finding Institution | Hope College |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 35°C |
GPS Coordinates | 42.35426 N, 86.28723 W Map |
Discovery Notes | Soil sample was collected on 9/3/22 @ 5:55 PM. The soil was damp and spongy and was collected approximately 3 inches down into a rotting tree stump in a nature preserve. The air temperature at the time was 79°F. |
Naming Notes | This phage is named after my high school chemistry teacher. His enthusiasm for science still inspires me every day. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Dec 13, 2022 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 868 |
Genome length (bp) | 54395 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CCGATGGCAT |
GC Content | 61.3% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | F |
Subcluster | F1 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
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Annotating Institution | Hope College |
Annotation Status | In GenBank |
Plaque Notes | The initial morphology after 24 hours of incubation is a clear plaque about 1 mm in size. However, after a week in storage at 4°C, the plaque will gain a crescent comet tail about 3mm in diameter. |
Has been Phamerated? | Yes |
Gene List |
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Submitted Annotation in DNA Master Format | Download |
Submitted Minimal DNA Master File | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | OR475252 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
151 |
Pitt Freezer Box Grid# |
H2 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
GenBank File for Phamerator | Download |