Mycobacterium phage Kenuha5
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Detailed Information for Phage Kenuha5
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByNur Ashykeen Mohd Fauzi
Year Found2017
Location FoundBoulder, CO USA
Finding InstitutionUniversity of Colorado at Boulder
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.012583 N, 105.269167 W Map
Discovery NotesThe soil sample was quite damp and collected somewhere at bushes near the Rec. Center of the University of Colorado Boulder.
Naming NotesThe name came from the combination of Nur's nickname "Ken" with her lab partner's "Nuha". The number 5 reflects that the phage was found at the fifth trial of bacteriophage enrichment.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 1, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage296
Genome length (bp)56502
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGAAGGCGC
GC Content61.7%
Sequencing NotesThis phage was sequenced as part of a mixed DOGEMS sample. The sequence of this genome was confirmed to belong to this phage by PCR.
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Renaud18 (None)

Alexphander (F1)

ArcusAngelus (F1)

Ardmore (F1)

Awesomesauce (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chuckly (F1)

Clifton (F1)

Cornucopia (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

Galactic (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Mozy (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sandalphon (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

Wachhund (F1)

Wee (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotating InstitutionUniversity of Colorado at Boulder
Annotation StatusQC Complete
Plaque NotesClear spots with slight turbid around the edges
Approximately 1-2nm
48 hours at 37°C
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Kenuha5_1
(terminase, small subunit)

Kenuha5_2
(terminase, large subunit)

Kenuha5_3
(portal protein)

Kenuha5_4
(capsid maturation protease)

Kenuha5_5
(scaffolding protein)

Kenuha5_6
(major capsid protein)

Kenuha5_7
(head-to-tail adaptor)

Kenuha5_8
(head-to-tail stopper)

Kenuha5_9

Kenuha5_10
(tail terminator)

Kenuha5_11
(major tail protein)

Kenuha5_12
(tail assmembly chaperone)

Kenuha5_13
(tail assembly chaperone)

Kenuha5_14
(tape measrue protein)

Kenuha5_15
(minor tail protein)

Kenuha5_16
(minor tail protein)

Kenuha5_17

Kenuha5_18
(minor tail protein)

Kenuha5_19
(minor tail protein D-ala-D-ala carboxypeptidase)

Kenuha5_20
(minor tail protein)

Kenuha5_21

Kenuha5_22
(helix-turn-helix dna binding domain)

Kenuha5_23

Kenuha5_24

Kenuha5_25

Kenuha5_26

Kenuha5_27
(lysin A)

Kenuha5_28
(Lysin B)

Kenuha5_29

Kenuha5_30
(membrane protein)

Kenuha5_31

Kenuha5_32
(DNA Q-like DNA polymerase III subunit)

Kenuha5_33

Kenuha5_34
(membrane protein)

Kenuha5_35
(helix-turn-helix dna binding domain)

Kenuha5_36

Kenuha5_37

Kenuha5_38

Kenuha5_39
(tyrosine integrase)

Kenuha5_40
(Hypotheical Protein)

Kenuha5_41
(immunity repressor)

Kenuha5_42
(cro (control of repressor's operator))

Kenuha5_43

Kenuha5_44

Kenuha5_45
(Hypotheical Protein)

Kenuha5_46
(Hypotheical Protein)

Kenuha5_47

Kenuha5_48
(excise)

Kenuha5_49

Kenuha5_50
(Hypotheical Protein)

Kenuha5_51

Kenuha5_52

Kenuha5_53

Kenuha5_54

Kenuha5_55
(WhiB family transcription factor)

Kenuha5_56
(helix-trun-helix DNA binding domain)

Kenuha5_57
(helix-turn-helix DNA binding domain)

Kenuha5_58

Kenuha5_59

Kenuha5_60

Kenuha5_61
(Ku-like dsDNA break-binding protein)

Kenuha5_62
(recA-like DNA recombinase)

Kenuha5_63
(HNH endonculease)

Kenuha5_64
(DNA helicase)

Kenuha5_65

Kenuha5_66
(DNA methylase)

Kenuha5_67
(ribbon helix-turn-helix DNA binding domain)

Kenuha5_68

Kenuha5_69

Kenuha5_70

Kenuha5_71

Kenuha5_72
(helix-turn-helix DNA binding domain)

Kenuha5_73

Kenuha5_74

Kenuha5_75

Kenuha5_76

Kenuha5_77

Kenuha5_78

Kenuha5_79

Kenuha5_80

Kenuha5_81

Kenuha5_82

Kenuha5_83

Kenuha5_84

Kenuha5_85

Kenuha5_86

Kenuha5_87
(helix-turn-helix DNA binding domain)

Kenuha5_88

Kenuha5_89

Kenuha5_90

Kenuha5_91

Kenuha5_92

Kenuha5_93
(glycosytransferase)

Kenuha5_94

Kenuha5_95
(glucosyltransferase)

Kenuha5_96
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 62
Pitt Freezer Box Grid# G7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload