Mycobacterium phage Moonbeam
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Detailed Information for Phage Moonbeam
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCassandra Michaels
Year Found2017
Location FoundButte, MT USA
Finding InstitutionHelena High School
ProgramMontana Tech Bringing Research Into the Classroom
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates46.5891 N, 112.0391 W Map
Discovery NotesDiscovered under the BRIC program at Montana Tech.
Genome annotation performed by Lauren Schmitt, Ozzie Ireland, and Abbie George.
SURF- amplification and DNA extraction by Hannah Sparks.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 3, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2558
Genome length (bp)57984
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGAAGGCGC
GC Content61.1%
Sequencing NotesThe sequencing reads indicate that in ~60% of the population of phages from which DNA was extracted, there was a 587 bp deletion. It runs from 25659 to 26245 (inclusive). Despite the majority of the population being this shorter version, the final fasta contains the deleted portion since otherwise it would be lost.
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Soul22 (None)

Akhila (F1)

Alexphander (F1)

ArcusAngelus (F1)

Ardmore (F1)

Awesomesauce (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilanaBonita (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sandalphon (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

Wachhund (F1)

Wee (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotating InstitutionHelena High School
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Moonbeam_1
(terminase, small subunit)

Moonbeam_2
(terminase, large subunit)

Moonbeam_3
(portal protein)

Moonbeam_4
(capsid maturation protease)

Moonbeam_5
(scaffolding protein)

Moonbeam_6
(major capsid protein)

Moonbeam_7
(head-to-tail adaptor)

Moonbeam_8
(head-to-tail stopper)

Moonbeam_9

Moonbeam_10
(tail terminator)

Moonbeam_11
(major tail protein)

Moonbeam_12
(tail assembly chaperone)

Moonbeam_13
(tail assembly chaperone)

Moonbeam_14
(HNH endonuclease)

Moonbeam_15
(tape measure protein)

Moonbeam_16
(minor tail protein)

Moonbeam_17
(minor tail protein)

Moonbeam_18
(minor tail protein)

Moonbeam_19
(minor tail protein)

Moonbeam_20
(minor tail protein, D-ala-D-ala carboxypeptidase)

Moonbeam_21
(minor tail protein)

Moonbeam_22

Moonbeam_23

Moonbeam_24

Moonbeam_25

Moonbeam_26
(helix-turn-helix DNA binding domain)

Moonbeam_27

Moonbeam_28

Moonbeam_29

Moonbeam_30

Moonbeam_31
(lysin A)

Moonbeam_32
(lysin B)

Moonbeam_33

Moonbeam_34

Moonbeam_35

Moonbeam_36
(DnaQ-like (DNA polymerase III subunit))

Moonbeam_37

Moonbeam_38

Moonbeam_39

Moonbeam_40

Moonbeam_41
(tyrosine integrase)

Moonbeam_42

Moonbeam_43
(immunity repressor)

Moonbeam_44

Moonbeam_45

Moonbeam_46
(excise)

Moonbeam_47

Moonbeam_48

Moonbeam_49

Moonbeam_50

Moonbeam_51

Moonbeam_52

Moonbeam_53

Moonbeam_54

Moonbeam_55

Moonbeam_56
(WhiB family transcription factor)

Moonbeam_57

Moonbeam_58
(helix-turn-helix DNA binding domain)

Moonbeam_59

Moonbeam_60

Moonbeam_61

Moonbeam_62

Moonbeam_63
(AAA-ATPase)

Moonbeam_64
(HNH endonuclease)

Moonbeam_65
(DNA helicase)

Moonbeam_66
(DNA methylase)

Moonbeam_67

Moonbeam_68

Moonbeam_69
(methyltransferase)

Moonbeam_70

Moonbeam_71

Moonbeam_72

Moonbeam_73

Moonbeam_74

Moonbeam_75

Moonbeam_76

Moonbeam_77

Moonbeam_78

Moonbeam_79

Moonbeam_80

Moonbeam_81

Moonbeam_82

Moonbeam_83

Moonbeam_84

Moonbeam_85

Moonbeam_86

Moonbeam_87

Moonbeam_88

Moonbeam_89

Moonbeam_90

Moonbeam_91

Moonbeam_92

Moonbeam_93

Moonbeam_94

Moonbeam_95

Moonbeam_96

Moonbeam_97
(helix-turn-helix DNA binding domain)

Moonbeam_98

Moonbeam_99

Moonbeam_100

Moonbeam_101

Moonbeam_102

Moonbeam_103

Moonbeam_104
(glycosyltransferase)

Moonbeam_105

Moonbeam_106
(glycosyltransferase)

Moonbeam_107
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT684593
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 92
Pitt Freezer Box Grid# A7
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload