Mycobacterium phage DaWorst
DaWorst Plaque Picture
DaWorst EM Picture

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Detailed Information for Phage DaWorst
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJack Collora
Year Found2016
Location FoundRiverside, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS CoordinatesUnavailable
Discovery NotesPhage DaWorst was isolated from somewhat moist dark brown soil, from a depth of 2-3cm on 10/1/16 by Jack Collora. Taken from Orange Fields in Citrus State park with permission. Air temp was 28C at time of collection. DaWorst was isolated using an enrichment culture incubated at 30C.

Characterization and annotation were performed by Team Kanye Western Blot (Danielle Abubakkar, Jack Collora, Alessandra Corrao, Jordan Ross) and Team Make Bacteria Great Again (Jonathan Zhu, Martin Ramirez, Bryan Chau, Ivan Perez). Annotation QC by Andrew Lund.

Instructor: Jordan Moberg-Parker
Teaching Assistants: Ximena Ibarra and Andrew Lund
Undergraduate Assistant: Hayley Ennis
Naming NotesIt was named "DaWorst" because we found it entertaining to refer to things using this name. Examples include "DaWorst plaques are clear and 3mm" and "DaWorst gene...".
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 20, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage106
Genome length (bp)56827
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCCGATGGCAT
GC Content61.0%
Fasta file available?Available upon entering GPS coordinates
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Lysogeny NotesTemperate phage. Large turbid plaques at higher temperatures. Annotation revealed presence of lysogeny genes such as excise, integrase, antirepressor, and immunity repressor.
Other Cluster Members

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Renaud18 (None)

Alexphander (F1)

ArcusAngelus (F1)

Ardmore (F1)

Awesomesauce (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chuckly (F1)

Clifton (F1)

Cornucopia (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

Galactic (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

Mahavrat (F1)

Mantra (F1)

Mattes (F1)

Melissauren88 (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Mozy (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sandalphon (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

Wachhund (F1)

Wee (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Zerg (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

TChen (F4)

ThetaBob (F4)

Cornie (F5)
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesClear at 30C.
Average size (and size range) diameter in mm: 3.0mm+/- 1mm at 30C

At >35C: halo appears. 6mm turbid plaque with 2mm clear centers.
MorphotypeSiphoviridae
Number of Genes103
Has been Phamerated?Yes
Gene List

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DaWorst_1
(HNH endonuclease)

DaWorst_2
(terminase small subunit)

DaWorst_3
(terminase large subunit)

DaWorst_4
(portal protein)

DaWorst_5
(capsid maturation protease)

DaWorst_6
(scaffolding protein)

DaWorst_7
(major capsid protein)

DaWorst_8
(head-to-tail adaptor)

DaWorst_9
(head-to-tail stopper)

DaWorst_10

DaWorst_11
(tail terminator)

DaWorst_12
(major tail protein)

DaWorst_13
(tail assembly chaperone)

DaWorst_14
(tail assembly chaperone)

DaWorst_15
(HNH endonuclease)

DaWorst_16
(tapemeasure protein)

DaWorst_17
(minor tail protein)

DaWorst_18
(minor tail protein)

DaWorst_19
(minor tail protein)

DaWorst_20
(minor tail protein)

DaWorst_21
(minor tail protein)

DaWorst_22
(minor tail protein)

DaWorst_23
(minor tail protein)

DaWorst_24
(minor tail protein)

DaWorst_25
(minor tail protein)

DaWorst_26
(minor tail protein)

DaWorst_27
(helix-turn-helix DNA binding domain protein)

DaWorst_28

DaWorst_29

DaWorst_30

DaWorst_31

DaWorst_32
(lysin A)

DaWorst_33
(lysin B)

DaWorst_34
(holin)

DaWorst_35
(membrane protein)

DaWorst_36

DaWorst_37
(DNA polymerase III subunit)

DaWorst_38

DaWorst_39

DaWorst_40

DaWorst_41

DaWorst_42

DaWorst_43
(MerR-like HTH DNA binding protein)

DaWorst_44

DaWorst_45
(integrase)

DaWorst_46

DaWorst_47
(immunity repressor)

DaWorst_48
(CRO protein)

DaWorst_49
(antirepressor)

DaWorst_50

DaWorst_51

DaWorst_52

DaWorst_53

DaWorst_54

DaWorst_55

DaWorst_56

DaWorst_57

DaWorst_58
(WhiB family transcription factor)

DaWorst_59

DaWorst_60
(WhiB family transcription factor)

DaWorst_61
(helix-turn-helix DNA binding domain protein)

DaWorst_62

DaWorst_63

DaWorst_64

DaWorst_65
(HNH endonuclease)

DaWorst_66
(DNA helicase)

DaWorst_67
(DNA methylase)

DaWorst_68

DaWorst_69

DaWorst_70

DaWorst_71

DaWorst_72

DaWorst_73

DaWorst_74
(helix-turn-helix DNA binding domain protein)

DaWorst_75

DaWorst_76

DaWorst_77

DaWorst_78

DaWorst_79

DaWorst_80

DaWorst_81

DaWorst_82

DaWorst_83

DaWorst_84

DaWorst_85

DaWorst_86

DaWorst_87

DaWorst_88

DaWorst_89

DaWorst_90

DaWorst_91

DaWorst_92

DaWorst_93

DaWorst_94
(MerR-like HTH DNA binding protein)

DaWorst_95

DaWorst_96

DaWorst_97

DaWorst_98

DaWorst_99

DaWorst_100
(glycosyltransferase)

DaWorst_101
(glycosyltransferase)

DaWorst_102

DaWorst_103
(glycosyltransferase)
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF919501
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 45
Pitt Freezer Box Grid# D3
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload