| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jack Collora |
| Year Found | 2016 |
| Location Found | Riverside, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.897238 N, 117.421712 W Map |
| Discovery Notes | Phage DaWorst was isolated from somewhat moist dark brown soil, from a depth of 2-3cm on 10/1/16 by Jack Collora. Taken from Orange Fields in Citrus State park with permission. Air temp was 28C at time of collection. DaWorst was isolated using an enrichment culture incubated at 30C.
Characterization and annotation were performed by Team Kanye Western Blot (Danielle Abubakkar, Jack Collora, Alessandra Corrao, Jordan Ross) and Team Make Bacteria Great Again (Jonathan Zhu, Martin Ramirez, Bryan Chau, Ivan Perez). Annotation QC by Andrew Lund.
Instructor: Jordan Moberg-Parker
Teaching Assistants: Ximena Ibarra and Andrew Lund
Undergraduate Assistant: Hayley Ennis |
| Naming Notes | It was named "DaWorst" because we found it entertaining to refer to things using this name. Examples include "DaWorst plaques are clear and 3mm" and "DaWorst gene...". |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 20, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 106 |
| Genome length (bp) | 56827 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CCGATGGCAT |
| GC Content | 61.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Temperate phage. Large turbid plaques at higher temperatures. Annotation revealed presence of lysogeny genes such as excise, integrase, antirepressor, and immunity repressor. |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Clear at 30C.
Average size (and size range) diameter in mm: 3.0mm+/- 1mm at 30C
At >35C: halo appears. 6mm turbid plaque with 2mm clear centers. |
| Morphotype | Siphoviridae |
| Number of Genes | 103 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF919501 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
45 |
| Pitt Freezer Box Grid# |
D3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
