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Detailed Information for Phage DaWorst
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Jack Collora
Year Found	2016
Location Found	Riverside, CA United States
Finding Institution	University of California, Los Angeles
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	30°C
GPS Coordinates	Unavailable
Discovery Notes	Phage DaWorst was isolated from somewhat moist dark brown soil, from a depth of 2-3cm on 10/1/16 by Jack Collora. Taken from Orange Fields in Citrus State park with permission. Air temp was 28C at time of collection. DaWorst was isolated using an enrichment culture incubated at 30C. Characterization and annotation were performed by Team Kanye Western Blot (Danielle Abubakkar, Jack Collora, Alessandra Corrao, Jordan Ross) and Team Make Bacteria Great Again (Jonathan Zhu, Martin Ramirez, Bryan Chau, Ivan Perez). Annotation QC by Andrew Lund. Instructor: Jordan Moberg-Parker Teaching Assistants: Ximena Ibarra and Andrew Lund Undergraduate Assistant: Hayley Ennis
Naming Notes	It was named "DaWorst" because we found it entertaining to refer to things using this name. Examples include "DaWorst plaques are clear and 3mm" and "DaWorst gene...".
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Dec 20, 2016
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	106
Genome length (bp)	56827
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CCGATGGCAT
GC Content	61.0%
Fasta file available?	Available upon entering GPS coordinates
Characterization
Cluster	F
Subcluster	F1
Cluster Life Cycle	Temperate
Lysogeny Notes	Temperate phage. Large turbid plaques at higher temperatures. Annotation revealed presence of lysogeny genes such as excise, integrase, antirepressor, and immunity repressor.
Other Cluster Members	Click to View AbbyNormal (None) Grib (None) IchabodCrane (None) Jiminy (None) Kishmeesh (None) KitCat (None) MightyMorph (None) Puck (None) Renaud18 (None) Sargento (None) WIVsmall1220 (None) Alexphander (F1) Anaxagorus (F1) Ant1Dodson (F1) ArcusAngelus (F1) Ardmore (F1) Arienne (F1) Asmart (F1) Awesomesauce (F1) BabytheFifth (F1) Barson (F1) Batiatus (F1) BigPhil (F1) Bipolar (F1) Blexus (F1) BobaPhett (F1) Bobi (F1) BodEinwohner17 (F1) BoomCrackaPop (F1) Boomer (F1) Braemar (F1) Brocalys (F1) Bubbles123 (F1) Burwell21 (F1) BuzzLyseyear (F1) ByChance (F1) Byougenkin (F1) Cabrinians (F1) Cakepop (F1) CaptainTrips (F1) Cerasum (F1) Che8 (F1) Chhibber (F1) Chikondi (F1) Chuckly (F1) Clifton (F1) Clover (F1) CoolGuy (F1) Cornucopia (F1) Daenerys (F1) Danda (F1) Dante (F1) DaWorst (F1) DCrust15 (F1) DeadP (F1) Desmond (F1) DillTech15 (F1) DirtMcgirt (F1) DLane (F1) Docouto (F1) Dorothy (F1) DotProduct (F1) Drago (F1) DRBy19 (F1) Eish (F1) EleanorGeorge (F1) Emma (F1) Empress (F1) Enby (F1) Estave1 (F1) Estella (F1) Fancypants (F1) Filuzino (F1) Firehouse51 (F1) Flathead (F1) Florinda (F1) Frankie (F1) Freddie4 (F1) Fruitloop (F1) FunOne (F1) Galactic (F1) Gardregs (F1) Gendisarray (F1) Genesis (F1) Geralt (F1) Girafales (F1) Girr (F1) Gorge (F1) GreatPumpkin (F1) GUmbie (F1) Hades (F1) Hamulus (F1) Harley (F1) Hegedechwinu (F1) Ibhubesi (F1) Inventum (F1) IrishSherpFalk (F1) James (F1) Job42 (F1) JoeyJr (F1) Judylove (F1) Juice456 (F1) Kalikimaka (F1) Katya (F1) Kenuha5 (F1) Kersh (F1) Kimberlium (F1) KingMidas (F1) Kingsley (F1) Koella (F1) Krakatau (F1) KristaRAM (F1) Latretium (F1) LillyLatzon (F1) LilMoolah (F1) Lizziana (F1) Llama (F1) Llij (F1) Lorde (F1) Lysinator (F1) MacDrage (F1) Mahavrat (F1) Mantra (F1) Mattes (F1) McDiddy (F1) Melissauren88 (F1) MerrillCO18 (F1) Mille (F1) MilleniumForce (F1) MinionDave (F1) Minnie (F1) Misha28 (F1) MisterCuddles (F1) Modragons (F1) Mozy (F1) Ms6 (F1) Mutaforma13 (F1) Myosotis (F1) Nemain (F1) Nimbo (F1) Nitzel (F1) Nivrat (F1) Noodle (F1) NormanBulbieJr (F1) Ochi17 (F1) OfUltron (F1) Ogopogo (F1) OldBen (F1) OlympiaSaint (F1) Ovechkin (F1) OwlsT2W (F1) Pacc40 (F1) Peanut21s (F1) Phabio (F1) PHappiness (F1) Phasih (F1) Phatniss (F1) PhrankthaTank (F1) Piper2020 (F1) Pippy (F1) Plackberry (F1) Plumbus (F1) PMC (F1) Poenanya (F1) Polka14 (F1) Pollywog (F1) PopTart (F1) Priscilla (F1) Prometheus (F1) QuickMath (F1) Quico (F1) Ramsey (F1) RitaG (F1) Rocktrap (F1) RockyHorror (F1) Rombolo (F1) Royals2015 (F1) Ruby (F1) S2Kari (F1) S2TSquared (F1) Saal (F1) SaltyMike (F1) Sandalphon (F1) SassyB (F1) Scottish (F1) Seagreen (F1) SG4 (F1) Sharpshooter (F1) Shauna1 (F1) SherL (F1) ShiLan (F1) SimranZ1 (F1) SiSi (F1) Sparkdehlily (F1) Spartacus (F1) Spikelee (F1) Spoonbill (F1) Strokeseat (F1) SuperGrey (F1) SwagPigglett (F1) Taj (F1) Taus (F1) TDanisky (F1) TonaldTDrump (F1) TootsiePop (F1) Tweety (F1) UncleRicky (F1) Velveteen (F1) Wachhund (F1) Wee (F1) WheatBerry18 (F1) Whouxphf (F1) WillSterrel (F1) WIVsmall (F1) XFactor (F1) Zerg (F1) Avani (F2) Che9d (F2) Demsculpinboyz (F2) Jabbawokkie (F2) Yoshi (F2) Zapner (F2) Squirty (F3) TChen (F4) ThetaBob (F4) Cornie (F5)
Annotation Status	In GenBank
Plaque Notes	Clear at 30C. Average size (and size range) diameter in mm: 3.0mm+/- 1mm at 30C At >35C: halo appears. 6mm turbid plaque with 2mm clear centers.
Morphotype	Siphoviridae
Number of Genes	103
Has been Phamerated?	Yes
Gene List	Click to View DaWorst_1 (HNH endonuclease) DaWorst_2 (terminase small subunit) DaWorst_3 (terminase large subunit) DaWorst_4 (portal protein) DaWorst_5 (capsid maturation protease) DaWorst_6 (scaffolding protein) DaWorst_7 (major capsid protein) DaWorst_8 (head-to-tail connector protein) DaWorst_9 (head-to-tail connector protein) DaWorst_10 (head-to-tail connector protein) DaWorst_11 (head-to-tail connector protein) DaWorst_12 (major tail protein) DaWorst_13 (tail assembly chaperone) DaWorst_14 (tail assembly chaperone) DaWorst_15 (HNH endonuclease) DaWorst_16 (tapemeasure protein) DaWorst_17 (minor tail protein) DaWorst_18 (minor tail protein) DaWorst_19 (minor tail protein) DaWorst_20 (minor tail protein) DaWorst_21 (minor tail protein) DaWorst_22 (minor tail protein) DaWorst_23 (minor tail protein) DaWorst_24 (minor tail protein) DaWorst_25 (minor tail protein) DaWorst_26 (minor tail protein) DaWorst_27 (helix-turn-helix DNA binding domain protein) DaWorst_28 DaWorst_29 DaWorst_30 DaWorst_31 DaWorst_32 (lysin A) DaWorst_33 (lysin B) DaWorst_34 (holin) DaWorst_35 (membrane protein) DaWorst_36 DaWorst_37 (DNA polymerase III subunit) DaWorst_38 DaWorst_39 DaWorst_40 DaWorst_41 DaWorst_42 DaWorst_43 (MerR-like HTH DNA binding protein) DaWorst_44 DaWorst_45 (integrase) DaWorst_46 DaWorst_47 (immunity repressor) DaWorst_48 (CRO protein) DaWorst_49 (antirepressor) DaWorst_50 DaWorst_51 DaWorst_52 DaWorst_53 DaWorst_54 DaWorst_55 DaWorst_56 DaWorst_57 DaWorst_58 (WhiB family transcription factor) DaWorst_59 DaWorst_60 (WhiB family transcription factor) DaWorst_61 (helix-turn-helix DNA binding domain protein) DaWorst_62 DaWorst_63 DaWorst_64 DaWorst_65 (HNH endonuclease) DaWorst_66 (DNA helicase) DaWorst_67 (DNA methylase) DaWorst_68 DaWorst_69 DaWorst_70 DaWorst_71 DaWorst_72 DaWorst_73 DaWorst_74 (helix-turn-helix DNA binding domain protein) DaWorst_75 DaWorst_76 DaWorst_77 DaWorst_78 DaWorst_79 DaWorst_80 DaWorst_81 DaWorst_82 DaWorst_83 DaWorst_84 DaWorst_85 DaWorst_86 DaWorst_87 DaWorst_88 DaWorst_89 DaWorst_90 DaWorst_91 DaWorst_92 DaWorst_93 DaWorst_94 (MerR-like HTH DNA binding protein) DaWorst_95 DaWorst_96 DaWorst_97 DaWorst_98 DaWorst_99 DaWorst_100 (glycosyltransferase) DaWorst_101 (glycosyltransferase) DaWorst_102 DaWorst_103 (glycosyltransferase)
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MF919501
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	45
Pitt Freezer Box Grid#	D3
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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