The Actinobacteriophage Database

Mycobacterium phage RockyHorror

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Detailed Information for Phage RockyHorror
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Janet Nickels
Year Found	2009
Location Found	Santa Cruz, CA USA
Finding Institution	University of California, Santa Cruz
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	36.991399 N, 122.060867 W Map
Discovery Notes	The soil collected was moist due to rain.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Mar 17, 2011
Sequencing Facility	Joint Genomics Institute
Genome length (bp)	56719
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
GC Content	61.1%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	F
Subcluster	F1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Renaud18 (None) Alexphander (F1) ArcusAngelus (F1) Ardmore (F1) Awesomesauce (F1) Batiatus (F1) BigPhil (F1) Bipolar (F1) Blexus (F1) BobaPhett (F1) Bobi (F1) BodEinwohner17 (F1) Boomer (F1) Brocalys (F1) Bubbles123 (F1) Burwell21 (F1) BuzzLyseyear (F1) ByChance (F1) Byougenkin (F1) Cabrinians (F1) CaptainTrips (F1) Cerasum (F1) Che8 (F1) Chuckly (F1) Clifton (F1) Cornucopia (F1) Daenerys (F1) Dante (F1) DaWorst (F1) DeadP (F1) DillTech15 (F1) DirtMcgirt (F1) DLane (F1) Donkeykong (F1) Doomphist (F1) Dorothy (F1) DotProduct (F1) Doug (F1) Drago (F1) DRBy19 (F1) Eish (F1) EleanorGeorge (F1) Emma (F1) Empress (F1) Enby (F1) Estave1 (F1) Fancypants (F1) Filuzino (F1) Firehouse51 (F1) Flathead (F1) Florinda (F1) Frankie (F1) Fruitloop (F1) Galactic (F1) Geralt (F1) Girafales (F1) Girr (F1) Gorge (F1) GUmbie (F1) Hades (F1) Hamulus (F1) Harley (F1) Hegedechwinu (F1) Ibhubesi (F1) Inventum (F1) IrishSherpFalk (F1) James (F1) Job42 (F1) JoeyJr (F1) Juice456 (F1) Kenuha5 (F1) Kersh (F1) Kimberlium (F1) KingMidas (F1) Kingsley (F1) Koella (F1) Krakatau (F1) KristaRAM (F1) LastJedi (F1) Latretium (F1) LilMoolah (F1) Lizziana (F1) Llama (F1) Llij (F1) Lorde (F1) Mahavrat (F1) Mantra (F1) Mattes (F1) Melissauren88 (F1) MilleniumForce (F1) MinionDave (F1) Minnie (F1) Misha28 (F1) MisterCuddles (F1) Modragons (F1) Mozy (F1) Mutaforma13 (F1) Nimbo (F1) Nitzel (F1) Nivrat (F1) NormanBulbieJr (F1) Ochi17 (F1) OfUltron (F1) Ogopogo (F1) OldBen (F1) OlympiaSaint (F1) Ovechkin (F1) OwlsT2W (F1) Pacc40 (F1) PHappiness (F1) Phasih (F1) Phatniss (F1) Piper2020 (F1) Pippy (F1) Plumbus (F1) PMC (F1) Poenanya (F1) Polka14 (F1) Pollywog (F1) PopTart (F1) Priscilla (F1) QuickMath (F1) Quico (F1) Ramsey (F1) RitaG (F1) RockyHorror (F1) Royals2015 (F1) Ruby (F1) Saal (F1) Sandalphon (F1) SassyB (F1) Scottish (F1) Seagreen (F1) SG4 (F1) Shauna1 (F1) ShiLan (F1) SimranZ1 (F1) SiSi (F1) Sparkdehlily (F1) Spartacus (F1) Spikelee (F1) Spoonbill (F1) StAnnes (F1) Strokeseat (F1) SuperGrey (F1) SwagPigglett (F1) Taj (F1) TDanisky (F1) TootsiePop (F1) Tweety (F1) UncleRicky (F1) Velveteen (F1) Wachhund (F1) Wee (F1) Whouxphf (F1) WillSterrel (F1) XFactor (F1) Zerg (F1) Avani (F2) Che9d (F2) Demsculpinboyz (F2) Jabbawokkie (F2) Yoshi (F2) Zapner (F2) Squirty (F3) TChen (F4) ThetaBob (F4) Cornie (F5)
Annotation Status	In GenBank
Plaque Notes	2.5mm clear plaques. 24 hour incubation period in 37oC conditions
Morphotype	Siphoviridae
Number of Genes	102
Has been Phamerated?	Yes
Gene List	Click to View RockyHorror_1 RockyHorror_2 RockyHorror_3 (terminase) RockyHorror_4 (portal protein) RockyHorror_5 (protease) RockyHorror_6 (scaffolding protein) RockyHorror_7 (major capsid protein) RockyHorror_8 RockyHorror_9 RockyHorror_10 RockyHorror_11 RockyHorror_12 (major tail subunit) RockyHorror_13 (tail assembly chaperone) RockyHorror_14 (tail assembly chaperone) RockyHorror_15 (tapemeasure protein) RockyHorror_16 RockyHorror_17 RockyHorror_18 RockyHorror_19 RockyHorror_20 RockyHorror_21 RockyHorror_22 RockyHorror_23 RockyHorror_24 RockyHorror_25 RockyHorror_26 RockyHorror_27 RockyHorror_28 RockyHorror_29 RockyHorror_30 RockyHorror_31 (lysin A) RockyHorror_32 (lysin B) RockyHorror_33 (holin) RockyHorror_34 RockyHorror_35 RockyHorror_36 (exonuclease) RockyHorror_37 RockyHorror_38 RockyHorror_39 RockyHorror_40 RockyHorror_41 RockyHorror_42 RockyHorror_43 RockyHorror_44 (integrase) RockyHorror_45 RockyHorror_46 RockyHorror_47 RockyHorror_48 RockyHorror_49 RockyHorror_50 RockyHorror_51 RockyHorror_52 RockyHorror_53 RockyHorror_54 RockyHorror_55 RockyHorror_56 RockyHorror_57 RockyHorror_58 RockyHorror_59 RockyHorror_60 RockyHorror_61 (WhiB) RockyHorror_62 RockyHorror_63 RockyHorror_64 RockyHorror_65 RockyHorror_66 RockyHorror_67 (DNA helicase) RockyHorror_68 RockyHorror_69 RockyHorror_70 RockyHorror_71 RockyHorror_72 RockyHorror_73 RockyHorror_74 RockyHorror_75 RockyHorror_76 RockyHorror_77 RockyHorror_78 RockyHorror_79 RockyHorror_80 RockyHorror_81 RockyHorror_82 RockyHorror_83 RockyHorror_84 RockyHorror_85 RockyHorror_86 RockyHorror_87 RockyHorror_88 RockyHorror_89 RockyHorror_90 RockyHorror_91 RockyHorror_92 RockyHorror_93 RockyHorror_94 RockyHorror_95 RockyHorror_96 RockyHorror_97 RockyHorror_98 RockyHorror_99 RockyHorror_100 RockyHorror_101 RockyHorror_102 RockyHorror_103 RockyHorror_104 (glycosyltransferase) RockyHorror_105 RockyHorror_106 RockyHorror_107
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	JF704117
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
SEA Lysate Titer	6e9
Pitt Freezer Box#	7
Pitt Freezer Box Grid#	2
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
Virtual Digest Picture	Download
EM Picture	Download

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