Mycobacterium phage Sassafras
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Detailed Information for Phage Sassafras
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJoshua Kammeraad
Year Found2010
Location FoundHolland, MI USA
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates42.789083 N, 86.104138 W Map
Discovery NotesSoil saturated with water, sample taken at a depth of 5cm, 22 degrees C, urban setting, flat
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 13, 2020
Sequencing FacilityHope College
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage123
Genome length (bp)57051
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGACGGCGC
GC Content61.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Soul22 (None)

Akhila (F1)

Alexphander (F1)

ArcusAngelus (F1)

Ardmore (F1)

Awesomesauce (F1)

Batiatus (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Eradicator (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

JalFarm20 (F1)

James (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

Leozinho (F1)

Leperchaun (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

MadMen (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilanaBonita (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Ramsey (F1)

RitaG (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sabbb (F1)

Sandalphon (F1)

Sarma624 (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tootsieroll (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

Wachhund (F1)

Wee (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Zerg (F1)

Zizzle (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusIn GenBank
Plaque NotesSassafras only produces one plaque morphology. Plaque diameters are only 1-2mm after 24 hours, but plaques grow steadily to over 1cm in diameter after a week of incubation. Low turbidity after 24 hours but then turbidity begins to range in a bulls-eye pattern that becomes more and then less turbid with distance from center.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Sassafras_1
(terminase, small subunit)

Sassafras_2
(terminase, large subunit)

Sassafras_3
(portal protein)

Sassafras_4
(capsid maturation protease)

Sassafras_5
(scaffolding protein)

Sassafras_6
(major capsid protein)

Sassafras_7
(head-to-tail adaptor)

Sassafras_8
(head-to-tail stopper)

Sassafras_9

Sassafras_10
(tail terminator)

Sassafras_11
(major tail protein)

Sassafras_12
(tail assembly chaperone)

Sassafras_13
(tail assembly chaperone)

Sassafras_14
(tape measure protein)

Sassafras_15
(minor tail protein)

Sassafras_16
(minor tail protein)

Sassafras_17
(minor tail protein)

Sassafras_18
(minor tail protein)

Sassafras_19
(minor tail protein, D-ala-D-ala carboxypeptidase)

Sassafras_20
(minor tail protein)

Sassafras_21
(minor tail protein)

Sassafras_22
(minor tail protein)

Sassafras_23
(minor tail protein)

Sassafras_24
(minor tail protein)

Sassafras_25
(helix-turn-helix DNA binding domain)

Sassafras_26

Sassafras_27

Sassafras_28

Sassafras_29

Sassafras_30
(lysin A)

Sassafras_31
(HNH endonuclease)

Sassafras_32
(lysin B)

Sassafras_33
(holin)

Sassafras_34

Sassafras_35

Sassafras_36
(DnaQ-like (DNA polymerase III subunit))

Sassafras_37

Sassafras_38

Sassafras_39

Sassafras_40

Sassafras_41
(excise)

Sassafras_42
(tyrosine integrase)

Sassafras_43

Sassafras_44

Sassafras_45
(immunity repressor)

Sassafras_46
(Cro (control of repressors operator))

Sassafras_47

Sassafras_48
(helix-turn-helix DNA binding domain)

Sassafras_49

Sassafras_50

Sassafras_51

Sassafras_52

Sassafras_53

Sassafras_54

Sassafras_55
(WhiB family transcription factor)

Sassafras_56
(helix-turn-helix DNA binding domain)

Sassafras_57
(WhiB family transcription factor)

Sassafras_58
(helix-turn-helix DNA binding domain)

Sassafras_59

Sassafras_60

Sassafras_61

Sassafras_62
(RecA-like DNA recombinase)

Sassafras_63

Sassafras_64
(HNH endonuclease)

Sassafras_65

Sassafras_66

Sassafras_67

Sassafras_68

Sassafras_69
(exonuclease)

Sassafras_70

Sassafras_71
(DNA methylase)

Sassafras_72
(DNA methylase)

Sassafras_73

Sassafras_74

Sassafras_75
(DNA methylase)

Sassafras_76

Sassafras_77

Sassafras_78

Sassafras_79

Sassafras_80

Sassafras_81

Sassafras_82

Sassafras_83

Sassafras_84

Sassafras_85

Sassafras_86

Sassafras_87

Sassafras_88

Sassafras_89

Sassafras_90

Sassafras_91

Sassafras_92

Sassafras_93

Sassafras_94

Sassafras_95

Sassafras_96
(helix-turn-helix DNA binding domain, MerR-like)

Sassafras_97

Sassafras_98

Sassafras_99

Sassafras_100

Sassafras_101

Sassafras_102

Sassafras_103
(glycosyltransferase)

Sassafras_104

Sassafras_105
(glycosyltransferase)

Sassafras_106
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMW862989
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2010HOPEsassafrasJAK
Pitt Freezer Box# 14
Pitt Freezer Box Grid# 30
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload