Mycobacterium phage Beakin
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Detailed Information for Phage Beakin
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByNatalia Quizena
Year Found2021
Location FoundGrandville, MI United States
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates42.88 N, 85.79 W Map
Discovery NotesThe phage was discovered in my backyard garden soil which contains cow manure, compost, and other organic and natural fertilizers such as kelp. Growing in the garden are tomatoes, cucumbers, radishes, and onions. Soil was obtained from the surface which was dry and from slightly below the surface which was slightly moist. The phage was discovered from both a direct plating and an enrichment spot test. Air temp 33°C.
Naming NotesThe phage is named after both of my parents, Beata and Elkin, and may also stand for the beacon of hope or "the Beakin of Hope"
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 5, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1167
Genome length (bp)54926
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCCGACGGCAT
GC Content61.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Lysogeny NotesBeakin shows lysogeny and has a lysogen created.
Other Cluster Members

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MulchSalad (None)

Soul22 (None)

Akhila (F1)

Alexphander (F1)

AlpineSix (F1)

ArcusAngelus (F1)

Ardmore (F1)

Aubs (F1)

Awesomesauce (F1)

Batiatus (F1)

Beakin (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BlueCrab (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

ChickenDinner (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

DaddyRickover (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Eradicator (F1)

Estave1 (F1)

Fancypants (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

GigiOuiOui (F1)

Girafales (F1)

Girr (F1)

GUmbie (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

Inventum (F1)

IrishSherpFalk (F1)

JalFarm20 (F1)

James (F1)

Jarcob (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Karhdo (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

Leozinho (F1)

Leperchaun (F1)

LilMoolah (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

LunaBlu (F1)

Madiba (F1)

MadMen (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilanaBonita (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Ms6 (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

Oksu (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phalconet (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Radiance (F1)

Ramsey (F1)

RedBird (F1)

Rialto (F1)

Rita (F1)

RitaG (F1)

Rockne (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sabbb (F1)

Sandalphon (F1)

Sarma624 (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

ShroomBoi (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Stap (F1)

Starcevich (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tootsieroll (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

VRedHorse (F1)

Wachhund (F1)

Wee (F1)

Whatsapiecost (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Yorick (F1)

Zerg (F1)

Zizzle (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Annotating InstitutionHope College
Annotation StatusIn GenBank
Plaque NotesThe features of the plaques are oval in shape with a murky ring on the outside and a clear oval in the inside of the plaque. Beakin plaques grow at 37 degrees C typically, however, are also able to grow at 42 degrees C.
Has been Phamerated?Yes
Gene List

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Beakin_1
(HNH endonuclease)

Beakin_2

Beakin_3
(terminase large subunit)

Beakin_4
(portal protein)

Beakin_5
(capsid maturation protease)

Beakin_6
(scaffolding protein)

Beakin_7
(major capsid protein)

Beakin_8
(head-to-tail adaptor)

Beakin_9
(head-to-tail stopper)

Beakin_10

Beakin_11
(tail terminator)

Beakin_12
(major tail protein)

Beakin_13
(tail assembly chaperone)

Beakin_14
(tail assembly chaperone)

Beakin_15
(tape measure protein)

Beakin_16
(minor tail protein)

Beakin_17
(minor tail protein)

Beakin_18
(minor tail protein)

Beakin_19
(minor tail protein)

Beakin_20
(minor tail protein)

Beakin_21
(minor tail protein)

Beakin_22

Beakin_23

Beakin_24

Beakin_25

Beakin_26

Beakin_27

Beakin_28

Beakin_29

Beakin_30

Beakin_31
(lysin A, N-acetylmuramoyl-L-alanine)

Beakin_32
(lysin B)

Beakin_33
(holin)

Beakin_34

Beakin_35

Beakin_36
(DnaQ-like DNA polymerase III subunit)

Beakin_37

Beakin_38

Beakin_39

Beakin_40

Beakin_41

Beakin_42

Beakin_43
(tyrosine integrase)

Beakin_44
(Rel3-like toxin)

Beakin_45
(RelB-like antitoxin)

Beakin_46

Beakin_47
(immunity repressor)

Beakin_48
(helix-turn-helix binding domain protein)

Beakin_49
(antirepressor)

Beakin_50

Beakin_51

Beakin_52
(excise)

Beakin_53

Beakin_54

Beakin_55

Beakin_56

Beakin_57

Beakin_58

Beakin_59
(WhiB family transcription factor)

Beakin_60

Beakin_61
(HTH DNA binding domain protein)

Beakin_62

Beakin_63

Beakin_64

Beakin_65
(ssDNA binding protein)

Beakin_66
(HNH endonuclease)

Beakin_67

Beakin_68

Beakin_69

Beakin_70

Beakin_71
(RepA-like replication initiator)

Beakin_72
(helicase loader)

Beakin_73
(DnaB-like dsDNA helicase)

Beakin_74
(holliday junction resolvase)

Beakin_75

Beakin_76
(MPME1 protein)

Beakin_77

Beakin_78

Beakin_79

Beakin_80

Beakin_81

Beakin_82

Beakin_83

Beakin_84
(G-I-Y Y-I-G endonuclease)

Beakin_85

Beakin_86
(MPME2 protein)

Beakin_87

Beakin_88

Beakin_89

Beakin_90

Beakin_91

Beakin_92

Beakin_93

Beakin_94
(glycosyltransferase)

Beakin_95
(serine/threorine kinase)

Beakin_96

Beakin_97
(glycosyltransferase)

Beakin_98

Beakin_99
(glycosyltransferase)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON970610
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 9.8x10^9
Pitt Freezer Box# 134
Pitt Freezer Box Grid# G2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload