| Detailed Information for Phage AlpineSix |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jack Maher |
| Year Found | 2019 |
| Location Found | West Lafayette, IN United States |
| Finding Institution | Purdue University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.432563 N, 86.932832 W Map |
| Discovery Notes | - soil was moist at the time found
- found near the roots of an alpine currant plant
-fertile soil with many bark particles present
- found 5.8-10.16cm below the surface with a 1-2in of a mulch layer above it |
| Naming Notes | It was named Alpine due to it being found near an alpine currant plant. The "six" part of the name came from it being our sixth trial in finding a phage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 13, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 842 |
| Genome length (bp) | 56721 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGATGGCGC |
| GC Content | 61.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Purdue University |
| Annotation Status | In GenBank |
| Plaque Notes | - small and circular plaques
- the plaques were very distinct in appearance
- had halos surrounding the plaques |
| Morphotype | Siphoviridae |
| Number of Genes | 102 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR475249 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
108 |
| Pitt Freezer Box Grid# |
A7 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
