| Detailed Information for Phage Awesomesauce |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Charlotte Merzbacher |
| Year Found | 2015 |
| Location Found | Providence, RI USA |
| Finding Institution | Brown University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.824593 N, 71.40338 W Map |
| Discovery Notes | The phage was isolated from damp soil found under the bushes on the north side of Everett-Poland House in Keeney Quad. It had not rained for several days, so this spot was ideal because it still held residual moisture from the last rain. The temperature was approximately 85 degrees Fahrenheit and the depth of the sample dug was 2 inches deep. |
| Naming Notes | Awesomesauce is truly an awesome phage, and it is bit saucy. I wanted my phage to feel empowered by its epic name. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 26, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2640 |
| Genome length (bp) | 57054 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CCGAAGGCAT |
| GC Content | 62.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | College of Charleston |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are generally 1-2 mm in diameter (depending on incubation period) and very clear; the phage is most likely lytic. |
| Morphotype | Siphoviridae |
| Number of Genes | 94 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT553347 |
| Refseq Number | None yet |
| Published in Paper | Davis et al 2020, Microbiology Resource Announcements: Genome Sequences of the Mycobacteriophages Awesomesauce and LastJedi |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| SEA Lysate Titer |
7.2x10^10 |
| Date of SEA Lysate Titering |
Oct 23, 2015 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
