| Detailed Information for Phage Beakin |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Natalia Quizena |
| Year Found | 2021 |
| Location Found | Grandville, MI United States |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 42.88 N, 85.79 W Map |
| Discovery Notes | The phage was discovered in my backyard garden soil which contains cow manure, compost, and other organic and natural fertilizers such as kelp. Growing in the garden are tomatoes, cucumbers, radishes, and onions. Soil was obtained from the surface which was dry and from slightly below the surface which was slightly moist. The phage was discovered from both a direct plating and an enrichment spot test. Air temp 33°C. |
| Naming Notes | The phage is named after both of my parents, Beata and Elkin, and may also stand for the beacon of hope or "the Beakin of Hope" |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 5, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1167 |
| Genome length (bp) | 54926 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CCGACGGCAT |
| GC Content | 61.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Beakin shows lysogeny and has a lysogen created. |
| Other Cluster Members |
|
| Annotating Institution | Hope College |
| Annotation Status | In GenBank |
| Plaque Notes | The features of the plaques are oval in shape with a murky ring on the outside and a clear oval in the inside of the plaque. Beakin plaques grow at 37 degrees C typically, however, are also able to grow at 42 degrees C. |
| Number of Genes | 99 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON970610 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
9.8x10^9 |
| Pitt Freezer Box# |
134 |
| Pitt Freezer Box Grid# |
G2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
