| Detailed Information for Phage Bipolar |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Monica Mulder and Shandee Tachick |
| Year Found | 2013 |
| Location Found | Caldwell, ID USA |
| Finding Institution | College of Idaho |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 43.655 N, 116.676389 W Map |
| Discovery Notes | The enrichment culture was found in front of the College of Idaho. |
| Naming Notes | We named it bipolarphage partly because sometimes the phage showed up clear, and sometimes it was hard to tell if it was a phage at all. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 4, 2014 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 235 |
| Sanger Finishing Reactions | 9 |
| Genome length (bp) | 58985 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGAAGGCGC |
| GC Content | 61.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 106 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KM597530 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Date of SEA Lysate Titering |
Oct 14, 2013 |
| Pitt Freezer Box# |
6 |
| Pitt Freezer Box Grid# |
G9 |
| Available Files |
| EM Picture | Download |
