| Detailed Information for Phage BobaPhett |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Annette Kang |
| Year Found | 2015 |
| Location Found | Philadelphia, PA USA |
| Finding Institution | Drexel University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.957689 N, 75.18926 W Map |
| Discovery Notes | Discovered within the deep moist depths of the Earth next to a bushbed near the Buckley Tennis Courts on the Drexel University Campus, Boba Phett had a rough childhood, having been brought up in a rough neighborhood surrounded by constant foot traffic and uncomfortable amounts of human perspiration and other unmentionable fluids excreted by the human body.
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| Naming Notes | A long time ago, in a galaxy far far away...existed Boba Phett, the ultimate bacteria bounty hunter. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 13, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1397 |
| Genome length (bp) | 59815 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGAAGGCGC |
| GC Content | 61.3% |
| Sequencing Notes | mixed sample, 16% reads Cluster B1 |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
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| Annotating Institution | Drexel University |
| Annotation Status | In GenBank |
| Plaque Notes | - approx. 3.5-4mm
- clear center with very slight haze surrounding the inner circumference of the phage
- moderately aggressively in terms of growth rate since the size is relatively small in comparison to how much bacterial lawn was cleared during each infection ( a silent but deadly killer)
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| Morphotype | Siphoviridae |
| Number of Genes | 108 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
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| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH155865 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| GenBank File for Phamerator | Download |