Mycobacterium phage Bobi
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Detailed Information for Phage Bobi
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMargaret Korty and Stephanie Maas
Year Found2010
Location FoundNorth Liberty, IN USA
Finding InstitutionPurdue University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates41.512536 N, 86.460357 W Map
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 23, 2012
Approximate Shotgun Coverage739
Genome length (bp)59179
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCCGATGGCAT
GC Content61.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Blarby (None)

MulchSalad (None)

Akhila (F1)

Alexphander (F1)

AlpineSix (F1)

ArcusAngelus (F1)

Ardmore (F1)

Aubs (F1)

Awesomesauce (F1)

Batiatus (F1)

Beakin (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BlueCrab (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chevrolet (F1)

ChickenDinner (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Crumble (F1)

DaddyRickover (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

Deb65 (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

DocMcStuffins (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DosHalletts (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Eradicator (F1)

Estave1 (F1)

Fancypants (F1)

Fastidio (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

FreddyB (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

GigiOuiOui (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Guppsters (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

IbOuu (F1)

IMpilo (F1)

Inventum (F1)

IrishSherpFalk (F1)

JalFarm20 (F1)

James (F1)

Jarcob (F1)

Jinglebell (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Juniper1 (F1)

Karhdo (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

Leozinho (F1)

Leperchaun (F1)

LilMoolah (F1)

LittleShirley (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

LunaBlu (F1)

Madiba (F1)

MadMen (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Maravista (F1)

Marker (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilanaBonita (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Ms6 (F1)

MulchExplorer (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

Oksu (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phalconet (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

PhesterPhotato (F1)

Philus (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Radiance (F1)

Ramsey (F1)

RedBird (F1)

Rialto (F1)

Rita (F1)

RitaG (F1)

Rockne (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sabbb (F1)

Sandalphon (F1)

Sarma624 (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

ShowerHandel (F1)

ShroomBoi (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Stap (F1)

Starcevich (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tootsieroll (F1)

Totinger (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

VRedHorse (F1)

Wachhund (F1)

Wee (F1)

Whatsapiecost (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Yorick (F1)

Zerg (F1)

Zizzle (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Soul22 (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

LunaStella (F4)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Toron (F6)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesVery small and slow growing plaques. The phage likes to shift plaque forms between platings. The general shape is a very small dot with a crescent shaped tail. The whole plaue usually doesn't measure more than 1mm.
MorphotypeSiphoviridae
Number of Genes107
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Bobi_1
(HNH endonuclease)

Bobi_2

Bobi_3
(terminase)

Bobi_4
(portal protein)

Bobi_5
(capsid maturation protease)

Bobi_6
(scaffolding protein)

Bobi_7
(major capsid protein)

Bobi_8
(head-to-tail adaptor)

Bobi_9
(head-to-tail stopper)

Bobi_10

Bobi_11
(tail terminator)

Bobi_12
(major tail subunit)

Bobi_13
(tail assembly chaperone)

Bobi_14
(tail assembly chaperone)

Bobi_15
(HNH endonuclease)

Bobi_16
(tape measure protein)

Bobi_17
(minor tail protein)

Bobi_18
(minor tail protein)

Bobi_19
(minor tail protein)

Bobi_20
(minor tail protein)

Bobi_21
(D-Ala-D-Ala carboxypeptidase)

Bobi_22

Bobi_23

Bobi_24

Bobi_25

Bobi_26

Bobi_27

Bobi_28

Bobi_29

Bobi_30

Bobi_31

Bobi_32
(LysA)

Bobi_33
(LysB)

Bobi_34
(holin)

Bobi_35

Bobi_36

Bobi_37
(DnaQ-like DNA polymerase III subunit)

Bobi_38

Bobi_39

Bobi_40

Bobi_41

Bobi_42

Bobi_43

Bobi_44

Bobi_45
(HNH endonuclease)

Bobi_46
(integrase)

Bobi_47

Bobi_48
(immunity repressor)

Bobi_49
(Cro protein)

Bobi_50

Bobi_51

Bobi_52

Bobi_53
(HNH endonuclease)

Bobi_54

Bobi_55

Bobi_56

Bobi_57

Bobi_58

Bobi_59

Bobi_60

Bobi_61

Bobi_62

Bobi_63
(WhiB)

Bobi_64

Bobi_65
(HTH DNA binding domain protein)

Bobi_66

Bobi_67

Bobi_68

Bobi_69
(HNH endonuclease)

Bobi_70

Bobi_71

Bobi_72

Bobi_73
(DNA methylase)

Bobi_74

Bobi_75
(DNA methylase)

Bobi_76

Bobi_77
(helix-turn-helix DNA binding domain protein)

Bobi_78

Bobi_79

Bobi_80

Bobi_81

Bobi_82

Bobi_83

Bobi_84

Bobi_85

Bobi_86

Bobi_87

Bobi_88

Bobi_89

Bobi_90

Bobi_91

Bobi_92

Bobi_93

Bobi_94

Bobi_95

Bobi_96

Bobi_97

Bobi_98

Bobi_99

Bobi_100

Bobi_101

Bobi_102
(glycosyltransferase)

Bobi_103

Bobi_104

Bobi_105

Bobi_106

Bobi_107
(glycosyltransferase)

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKF114874
Refseq NumberNC_022055
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator 2010PURDbobiMMKSM
SEA Lysate Titer 1.06e8
Date of SEA Lysate Titering Nov 10, 2010
Pitt Freezer Box# 16
Pitt Freezer Box Grid# 8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload