The Actinobacteriophage Database

Mycobacterium phage Bobi

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Detailed Information for Phage Bobi
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Margaret Korty and Stephanie Maas
Year Found	2010
Location Found	North Liberty, IN USA
Finding Institution	Purdue University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	41.512536 N, 86.460357 W Map
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Feb 23, 2012
Approximate Shotgun Coverage	739
Genome length (bp)	59179
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CCGATGGCAT
GC Content	61.7%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	F
Subcluster	F1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Blarby (None) MulchSalad (None) Akhila (F1) Alexphander (F1) AlpineSix (F1) ArcusAngelus (F1) Ardmore (F1) Aubs (F1) Awesomesauce (F1) Batiatus (F1) Beakin (F1) BigPhil (F1) Bipolar (F1) Blexus (F1) BlueCrab (F1) BobaPhett (F1) Bobi (F1) BodEinwohner17 (F1) Boomer (F1) Brocalys (F1) Bubbles123 (F1) Burwell21 (F1) BuzzLyseyear (F1) ByChance (F1) Byougenkin (F1) Cabrinians (F1) CaptainTrips (F1) Cerasum (F1) Che8 (F1) Chevrolet (F1) ChickenDinner (F1) Chuckly (F1) Clifton (F1) Coco12 (F1) Cornucopia (F1) Crumble (F1) DaddyRickover (F1) Daenerys (F1) Dante (F1) DaWorst (F1) DeadP (F1) Deb65 (F1) DillTech15 (F1) DirtMcgirt (F1) DLane (F1) DocMcStuffins (F1) Donkeykong (F1) Doomphist (F1) Dorothy (F1) DosHalletts (F1) DotProduct (F1) Doug (F1) Drago (F1) DRBy19 (F1) Eish (F1) EleanorGeorge (F1) Emma (F1) Empress (F1) Enby (F1) Eradicator (F1) Estave1 (F1) Fancypants (F1) Fastidio (F1) Filuzino (F1) Firehouse51 (F1) Flathead (F1) Florinda (F1) Frankie (F1) FreddyB (F1) Fruitloop (F1) Galactic (F1) Gandalph (F1) Geralt (F1) GigiOuiOui (F1) Girafales (F1) Girr (F1) Gorge (F1) GUmbie (F1) Guppsters (F1) Hades (F1) Hamulus (F1) Harley (F1) Hegedechwinu (F1) Hlubikazi (F1) Ibhubesi (F1) IbOuu (F1) Inventum (F1) IrishSherpFalk (F1) JalFarm20 (F1) James (F1) Jarcob (F1) Jinglebell (F1) Job42 (F1) JoeyJr (F1) Juice456 (F1) Juniper1 (F1) Karhdo (F1) Kenuha5 (F1) Kersh (F1) Kimberlium (F1) KingMidas (F1) Kingsley (F1) Koella (F1) Krakatau (F1) KristaRAM (F1) LastJedi (F1) Latretium (F1) Leozinho (F1) Leperchaun (F1) LilMoolah (F1) LittleShirley (F1) Lizziana (F1) Llama (F1) Llij (F1) Lorde (F1) LunaBlu (F1) Madiba (F1) MadMen (F1) Mahavrat (F1) Mandlovu (F1) Mantra (F1) Maravista (F1) Marker (F1) Martik (F1) Mattes (F1) Melissauren88 (F1) MilanaBonita (F1) MilleniumForce (F1) MinionDave (F1) Minnie (F1) Misha28 (F1) MisterCuddles (F1) Modragons (F1) Moonbeam (F1) Mova (F1) Mozy (F1) Ms6 (F1) Mutaforma13 (F1) Nimbo (F1) Nitzel (F1) Nivrat (F1) NormanBulbieJr (F1) Ochi17 (F1) OfUltron (F1) Ogopogo (F1) Oksu (F1) OldBen (F1) OlympiaSaint (F1) Ovechkin (F1) OwlsT2W (F1) Pacc40 (F1) Phalconet (F1) Phanphagia (F1) PHappiness (F1) Phasih (F1) Phatniss (F1) PhesterPhotato (F1) Philus (F1) Piper2020 (F1) Pippy (F1) Plumbus (F1) PMC (F1) Poenanya (F1) Polka14 (F1) Pollywog (F1) PopTart (F1) Priscilla (F1) QuickMath (F1) Quico (F1) Radiance (F1) Ramsey (F1) RedBird (F1) Rialto (F1) Rita (F1) RitaG (F1) Rockne (F1) RockyHorror (F1) Royals2015 (F1) Ruby (F1) Saal (F1) Sabbb (F1) Sandalphon (F1) Sarma624 (F1) Sassafras (F1) SassyB (F1) Scottish (F1) Seabastian (F1) Seagreen (F1) SG4 (F1) Shauna1 (F1) ShiLan (F1) ShowerHandel (F1) ShroomBoi (F1) SimranZ1 (F1) SiSi (F1) Sparkdehlily (F1) Spartacus (F1) Spikelee (F1) Spoonbill (F1) StAnnes (F1) Stap (F1) Starcevich (F1) Strokeseat (F1) SuperGrey (F1) SwagPigglett (F1) Taj (F1) TDanisky (F1) TootsiePop (F1) Tootsieroll (F1) Totinger (F1) Tweety (F1) UncleRicky (F1) Velveteen (F1) Veteran (F1) VRedHorse (F1) Wachhund (F1) Wee (F1) Whatsapiecost (F1) Whouxphf (F1) WillSterrel (F1) XFactor (F1) Yorick (F1) Zerg (F1) Zizzle (F1) Avani (F2) Che9d (F2) Demsculpinboyz (F2) Jabbawokkie (F2) Soul22 (F2) Yoshi (F2) Zapner (F2) Squirty (F3) LunaStella (F4) Renaud18 (F4) TChen (F4) ThetaBob (F4) Cornie (F5)
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	Very small and slow growing plaques. The phage likes to shift plaque forms between platings. The general shape is a very small dot with a crescent shaped tail. The whole plaue usually doesn't measure more than 1mm.
Morphotype	Siphoviridae
Number of Genes	107
Has been Phamerated?	Yes
Gene List	Click to View Bobi_1 (HNH endonuclease) Bobi_2 Bobi_3 (terminase) Bobi_4 (portal protein) Bobi_5 (capsid maturation protease) Bobi_6 (scaffolding protein) Bobi_7 (major capsid protein) Bobi_8 (head-to-tail adaptor) Bobi_9 (head-to-tail stopper) Bobi_10 Bobi_11 (tail terminator) Bobi_12 (major tail subunit) Bobi_13 (tail assembly chaperone) Bobi_14 (tail assembly chaperone) Bobi_15 (HNH endonuclease) Bobi_16 (tape measure protein) Bobi_17 (minor tail protein) Bobi_18 (minor tail protein) Bobi_19 (minor tail protein) Bobi_20 (minor tail protein) Bobi_21 (D-Ala-D-Ala carboxypeptidase) Bobi_22 Bobi_23 Bobi_24 Bobi_25 Bobi_26 Bobi_27 Bobi_28 Bobi_29 Bobi_30 Bobi_31 Bobi_32 (LysA) Bobi_33 (LysB) Bobi_34 (holin) Bobi_35 Bobi_36 Bobi_37 (DnaQ-like DNA polymerase III subunit) Bobi_38 Bobi_39 Bobi_40 Bobi_41 Bobi_42 Bobi_43 Bobi_44 Bobi_45 (HNH endonuclease) Bobi_46 (integrase) Bobi_47 Bobi_48 (immunity repressor) Bobi_49 (Cro protein) Bobi_50 Bobi_51 Bobi_52 Bobi_53 (HNH endonuclease) Bobi_54 Bobi_55 Bobi_56 Bobi_57 Bobi_58 Bobi_59 Bobi_60 Bobi_61 Bobi_62 Bobi_63 (WhiB) Bobi_64 Bobi_65 (HTH DNA binding domain protein) Bobi_66 Bobi_67 Bobi_68 Bobi_69 (HNH endonuclease) Bobi_70 Bobi_71 Bobi_72 Bobi_73 (DNA methylase) Bobi_74 Bobi_75 (DNA methylase) Bobi_76 Bobi_77 (helix-turn-helix DNA binding domain protein) Bobi_78 Bobi_79 Bobi_80 Bobi_81 Bobi_82 Bobi_83 Bobi_84 Bobi_85 Bobi_86 Bobi_87 Bobi_88 Bobi_89 Bobi_90 Bobi_91 Bobi_92 Bobi_93 Bobi_94 Bobi_95 Bobi_96 Bobi_97 Bobi_98 Bobi_99 Bobi_100 Bobi_101 Bobi_102 (glycosyltransferase) Bobi_103 Bobi_104 Bobi_105 Bobi_106 Bobi_107 (glycosyltransferase)
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	KF114874
Refseq Number	NC_022055
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
SEA Designator	2010PURDbobiMMKSM
SEA Lysate Titer	1.06e8
Date of SEA Lysate Titering	Nov 10, 2010
Pitt Freezer Box#	16
Pitt Freezer Box Grid#	8
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download

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