| Detailed Information for Phage Brookers |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Megan Howard |
| Year Found | 2024 |
| Location Found | Provo, UT USA |
| Finding Institution | Brigham Young University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 21°C |
| GPS Coordinates | 40.239736 N, 111.657426 W Map |
| Discovery Notes | Soil sample was taken from a terrarium, however soil from terrarium was taken from GPS coordinates originally. When sample of soil was taken from terrarium, it was at room temperature as noted above. |
| Naming Notes | Megan Howard was collecting multiple samples, one included outside of an ice cream place called "Brookers". Megan liked the name and decided to stick with it, even though her phage was not found there. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 13, 2025 |
| Sequencing Facility | CD Genomics |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NovaSeq X Plus |
| Read Type | Paired-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 4630 |
| Genome length (bp) | 56569 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CCGATGGCAT |
| GC Content | 61.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Brigham Young University |
| Annotation Status | QC Complete |
| Plaque Notes | Phage during isolation has taken form in two ways, smaller plaques, and bigger plaques. The original phage was larger, about 5mm in radius. |
| Morphotype | Siphoviridae |
| Number of Genes | 102 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
209 |
| Pitt Freezer Box Grid# |
A1 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
