| Detailed Information for Phage Brushbloom |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Adeline John & Sameen Hossain |
| Year Found | 2024 |
| Location Found | Richmond, VA USA |
| Finding Institution | Virginia Commonwealth University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 37.545056 N, 77.454783 W Map |
| Discovery Notes | GPS: In front of Trani Life Sciences Building, VCU Monroe Park Campus
Physical Description: Wet and Clay-Like Soil
Collection Date: September 17th, 2024 |
| Naming Notes | Named after the plant Dwarf fothergilla, as the flower looks like a brush and blooms outward. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 10, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 10948 |
| Genome length (bp) | 58749 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CCGATGGCAT |
| GC Content | 61.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Virginia Commonwealth University |
| Annotation Status | QC Complete |
| Plaque Notes | Clear small round and in 0.5 mm in diameter |
| Morphotype | Siphoviridae |
| Number of Genes | 106 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.5X10^10 |
| Pitt Freezer Box# |
200 |
| Pitt Freezer Box Grid# |
G10 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
