The Actinobacteriophage Database

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Detailed Information for Phage Bubbles123
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Sarah Torres and Paul Merlau
Year Found	2012
Location Found	Grand Prairie, TX USA
Finding Institution	University of North Texas
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	32.588889 N, 97.041944 W Map
Naming Notes	The phage resembles a bubble when held up to the light through its unique reflection and spectrum of colors.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Dec 19, 2013
Sequencing Facility	University of North Texas
Shotgun Sequencing Method	Ion Torrent
Approximate Shotgun Coverage	335
Genome length (bp)	57647
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CGGTAGGCGC
GC Content	61.4%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	F
Subcluster	F1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Blarby (None) MulchSalad (None) Akhila (F1) Alexphander (F1) AlpineSix (F1) ArcusAngelus (F1) Ardmore (F1) Aubs (F1) Awesomesauce (F1) Batiatus (F1) Beakin (F1) BigPhil (F1) Bipolar (F1) Blexus (F1) BlueCrab (F1) BobaPhett (F1) Bobi (F1) BodEinwohner17 (F1) Boomer (F1) Brocalys (F1) Bubbles123 (F1) Burwell21 (F1) BuzzLyseyear (F1) ByChance (F1) Byougenkin (F1) Cabrinians (F1) CaptainTrips (F1) Cerasum (F1) Che8 (F1) Chevrolet (F1) ChickenDinner (F1) Chuckly (F1) Clifton (F1) Coco12 (F1) Cornucopia (F1) Crumble (F1) DaddyRickover (F1) Daenerys (F1) Dante (F1) DaWorst (F1) DeadP (F1) Deb65 (F1) DillTech15 (F1) DirtMcgirt (F1) DLane (F1) DocMcStuffins (F1) Donkeykong (F1) Doomphist (F1) Dorothy (F1) DosHalletts (F1) DotProduct (F1) Doug (F1) Drago (F1) DRBy19 (F1) Eish (F1) EleanorGeorge (F1) Emma (F1) Empress (F1) Enby (F1) Eradicator (F1) Estave1 (F1) Fancypants (F1) Fastidio (F1) Filuzino (F1) Firehouse51 (F1) Flathead (F1) Florinda (F1) Frankie (F1) FreddyB (F1) Fruitloop (F1) Galactic (F1) Gandalph (F1) Geralt (F1) GigiOuiOui (F1) Girafales (F1) Girr (F1) Gorge (F1) GUmbie (F1) Guppsters (F1) Hades (F1) Hamulus (F1) Harley (F1) Hegedechwinu (F1) Hlubikazi (F1) Ibhubesi (F1) IbOuu (F1) IMpilo (F1) Inventum (F1) IrishSherpFalk (F1) JalFarm20 (F1) James (F1) Jarcob (F1) Jinglebell (F1) Job42 (F1) JoeyJr (F1) Juice456 (F1) Juniper1 (F1) Karhdo (F1) Kenuha5 (F1) Kersh (F1) Kimberlium (F1) KingMidas (F1) Kingsley (F1) Koella (F1) Krakatau (F1) KristaRAM (F1) LastJedi (F1) Latretium (F1) Leozinho (F1) Leperchaun (F1) LilMoolah (F1) LittleShirley (F1) Lizziana (F1) Llama (F1) Llij (F1) Lorde (F1) LunaBlu (F1) Madiba (F1) MadMen (F1) Mahavrat (F1) Mandlovu (F1) Mantra (F1) Maravista (F1) Marker (F1) Martik (F1) Mattes (F1) Melissauren88 (F1) MilanaBonita (F1) MilleniumForce (F1) MinionDave (F1) Minnie (F1) Misha28 (F1) MisterCuddles (F1) Modragons (F1) Moonbeam (F1) Mova (F1) Mozy (F1) Ms6 (F1) MulchExplorer (F1) Mutaforma13 (F1) Nimbo (F1) Nitzel (F1) Nivrat (F1) NormanBulbieJr (F1) Ochi17 (F1) OfUltron (F1) Ogopogo (F1) Oksu (F1) OldBen (F1) OlympiaSaint (F1) Ovechkin (F1) OwlsT2W (F1) Pacc40 (F1) Phalconet (F1) Phanphagia (F1) PHappiness (F1) Phasih (F1) Phatniss (F1) PhesterPhotato (F1) Philus (F1) Piper2020 (F1) Pippy (F1) Plumbus (F1) PMC (F1) Poenanya (F1) Polka14 (F1) Pollywog (F1) PopTart (F1) Priscilla (F1) QuickMath (F1) Quico (F1) Radiance (F1) Ramsey (F1) RedBird (F1) Rialto (F1) Rita (F1) RitaG (F1) Rockne (F1) RockyHorror (F1) Royals2015 (F1) Ruby (F1) Saal (F1) Sabbb (F1) Sandalphon (F1) Sarma624 (F1) Sassafras (F1) SassyB (F1) Scottish (F1) Seabastian (F1) Seagreen (F1) SG4 (F1) Shauna1 (F1) ShiLan (F1) ShowerHandel (F1) ShroomBoi (F1) SimranZ1 (F1) SiSi (F1) Sparkdehlily (F1) Spartacus (F1) Spikelee (F1) Spoonbill (F1) StAnnes (F1) Stap (F1) Starcevich (F1) Strokeseat (F1) SuperGrey (F1) SwagPigglett (F1) Taj (F1) TDanisky (F1) TootsiePop (F1) Tootsieroll (F1) Totinger (F1) Tweety (F1) UncleRicky (F1) Velveteen (F1) Veteran (F1) VRedHorse (F1) Wachhund (F1) Wee (F1) Whatsapiecost (F1) Whouxphf (F1) WillSterrel (F1) XFactor (F1) Yorick (F1) Zerg (F1) Zizzle (F1) Avani (F2) Che9d (F2) Demsculpinboyz (F2) Jabbawokkie (F2) Soul22 (F2) Yoshi (F2) Zapner (F2) Squirty (F3) LunaStella (F4) Renaud18 (F4) TChen (F4) ThetaBob (F4) Cornie (F5) Toron (F6)
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	The average size of the plaque is about 1 mm and resembles a near perfect circle.
Morphotype	Siphoviridae
Number of Genes	100
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Bubbles123_1 (terminase small subunit) Bubbles123_2 (terminase large subunit) Bubbles123_3 (portal protein) Bubbles123_4 (capsid maturation protease) Bubbles123_5 (scaffolding protein) Bubbles123_6 (major capsid protein) Bubbles123_7 (head-to-tail adaptor) Bubbles123_8 (head-to-tail stopper) Bubbles123_9 Bubbles123_10 (tail terminator) Bubbles123_11 (major tail protein) Bubbles123_12 (tail assembly chaperone) Bubbles123_13 (tail assembly chaperone) Bubbles123_14 (HNH endonuclease) Bubbles123_15 (tape measure protein) Bubbles123_16 (minor tail protein) Bubbles123_17 (minor tail protein) Bubbles123_18 (minor tail protein) Bubbles123_19 (minor tail protein) Bubbles123_20 (minor tail protein) Bubbles123_21 Bubbles123_22 Bubbles123_23 Bubbles123_24 (minor tail protein) Bubbles123_25 Bubbles123_26 (helix-turn-helix DNA binding domain protein) Bubbles123_27 Bubbles123_28 Bubbles123_29 Bubbles123_30 Bubbles123_31 Bubbles123_32 (lysin A) Bubbles123_33 (lysin B) Bubbles123_34 (holin) Bubbles123_35 Bubbles123_36 Bubbles123_37 (DnaQ-like DNA polymerase III subunit) Bubbles123_38 Bubbles123_39 Bubbles123_40 (tyrosine integrase) Bubbles123_41 Bubbles123_42 (immunity repressor) Bubbles123_43 (CRO protein) Bubbles123_44 (antirepressor) Bubbles123_45 Bubbles123_46 Bubbles123_47 (excise) Bubbles123_48 Bubbles123_49 Bubbles123_50 Bubbles123_51 Bubbles123_52 Bubbles123_53 Bubbles123_54 (WhiB family transcription factor) Bubbles123_55 Bubbles123_56 (helix-turn-helix DNA binding domain protein) Bubbles123_57 Bubbles123_58 Bubbles123_59 Bubbles123_60 Bubbles123_61 (HNH endonuclease) Bubbles123_62 (helicase) Bubbles123_63 (DNA methylase) Bubbles123_64 Bubbles123_65 Bubbles123_66 (DNA methylase) Bubbles123_67 Bubbles123_68 Bubbles123_69 (G-I-Y Y-I-G endonuclease) Bubbles123_70 Bubbles123_71 Bubbles123_72 Bubbles123_73 Bubbles123_74 Bubbles123_75 Bubbles123_76 Bubbles123_77 Bubbles123_78 Bubbles123_79 Bubbles123_80 Bubbles123_81 Bubbles123_82 Bubbles123_83 Bubbles123_84 Bubbles123_85 Bubbles123_86 Bubbles123_87 (G-I-Y Y-I-G endonuclease) Bubbles123_88 Bubbles123_89 Bubbles123_90 Bubbles123_91 Bubbles123_92 Bubbles123_93 Bubbles123_94 Bubbles123_95 (glycosyltransferase) Bubbles123_96 Bubbles123_97 Bubbles123_98 Bubbles123_99 Bubbles123_100 (HNH endonuclease)
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	KY348865
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	40
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
Final DNAMaster File	Download
GenBank File for Phamerator	Download

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