| Detailed Information for Phage ByChance |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Pallavi Kawatra |
| Year Found | 2017 |
| Location Found | Philadelphia, PA USA |
| Finding Institution | University of the Sciences (now St. Joseph's Univ) |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.9484 N, 75.20905 W Map |
| Discovery Notes | The soil sample I collected was from the University of the Sciences campus on September 12, 2017 at 6:35pm. I went very deep into the soil, and it was moist. It was around a plant, and there is not much foot traffic there. It is close to buildings, and appears to be taken care of regularly. The soil was very dark and moist. |
| Naming Notes | The name ByChance was selected because seeing my phage is a very "by chance" type situation. In the beginning, actually seeing the phage was rare; it was there, but very small plaques and very hidden. Even as time went on, ByChance was still rarely seen and is density dependent showing one of the highest concentrations on its fourth dilution. But when you see ByChance, it is a beautiful occasion marked with much joy. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 8, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1988 |
| Genome length (bp) | 53123 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGACGGCGC |
| GC Content | 61.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of the Sciences (now St. Joseph's Univ) |
| Annotation Status | In GenBank |
| Plaque Notes | The phage appears to be lytic as there are clear plaques on the plates. |
| Morphotype | Siphoviridae |
| Number of Genes | 95 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH399771 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
47 |
| Pitt Freezer Box Grid# |
B8 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
