| Detailed Information for Phage Cornie |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Justin Lugo |
| Year Found | 2016 |
| Location Found | Fayetteville, NC USA |
| Finding Institution | Fayetteville State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 35.01814 N, 78.895187 W Map |
| Discovery Notes | Weather was an overcast with cool temps in the low 70s. It had rain the previous evening. The location of the sample was the public vehicle area(PVA) water run off a Waffle House parking lot. The dumpster was at the other side of the PVA. There was several debris, ants, and litter. The top layer was moved and the sample was then taken. |
| Naming Notes | The name I chose was a childhood nickname. I use to make lame jokes and my friends called me out on it all the time. For several years my friends would only refer to me as Cornie. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 9, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1200 |
| Genome length (bp) | 56391 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CCCGGCCCA |
| GC Content | 61.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F5 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | The integrase gene of this phage is missing the N-terminus and is fused to the DNA pol gene upstream of it. We tried to generate lysogens and were unsuccessful. Therefore, we conclude that this integrase is non-functional. |
| Other Cluster Members |
|
| Annotating Institution | Rockland Community College |
| Annotation Status | In GenBank |
| Plaque Notes | Small (1mm) circular plaques with the edges appearing to defined. Sometimes displays short comet tails. |
| Morphotype | Siphoviridae |
| Number of Genes | 101 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN908688 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.3 x 10^-10 |
| Date of SEA Lysate Titering |
Nov 2, 2016 |
| Pitt Freezer Box# |
66 |
| Pitt Freezer Box Grid# |
A9 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
