| Detailed Information for Phage Crumble |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Hisham Naveed, Emerson Bavuso, Phuong-Anh Bui |
| Year Found | 2023 |
| Location Found | Richmond, VA United States of America |
| Finding Institution | Virginia Commonwealth University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 37.544917 N, 77.455 W Map |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 17, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 717 |
| Genome length (bp) | 53424 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGACGGCGC |
| GC Content | 61.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Virginia Commonwealth University |
| Annotation Status | In GenBank |
| Plaque Notes | Clear, circular plaques upon isolation. There appear to be both siphoviridae and myoviridae in the sample, as seen in the TEM image. |
| Morphotype | Siphoviridae |
| Number of Genes | 99 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP978871 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
10^10 pfu/ml |
| Pitt Freezer Box# |
185 |
| Pitt Freezer Box Grid# |
C7 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
