The Actinobacteriophage Database

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Detailed Information for Phage DRBy19
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Mia Wenzel
Year Found	2015
Location Found	Harrisonburg, VA USA
Finding Institution	James Madison University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Unknown
Isolation Temperature	Not entered
GPS Coordinates	38.426439 N, 78.859958 W Map
Naming Notes	I play roller derby, and my number is #19. So I chose to name my phage DRBy19, a small piece of who I am right now!
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 13, 2016
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Approximate Shotgun Coverage	1701
Genome length (bp)	58368
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CGGAAGGCGC
GC Content	61.3%
Sequencing Notes	There was a contaminating phage, present in ~10% of reads, that was another Cluster F1 phage. In addition, this phage has a number of apparent SNPs. They are: Positions 18863-4, ~38% of reads call GA instead of consensus CC. Position 20123, ~43% of reads call T instead of consensus G. Position 53499, ~48% of reads call C instead of consensus A. Position 53646, ~38% of reads call C instead of consensus T. Position 53715, ~50% of reads call C instead of consensus T.
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	F
Subcluster	F1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Blarby (None) MulchSalad (None) Akhila (F1) Alexphander (F1) AlpineSix (F1) ArcusAngelus (F1) Ardmore (F1) Aubs (F1) Awesomesauce (F1) Batiatus (F1) Beakin (F1) BigPhil (F1) Bipolar (F1) Blexus (F1) BlueCrab (F1) BobaPhett (F1) Bobi (F1) BodEinwohner17 (F1) Boomer (F1) Brocalys (F1) Bubbles123 (F1) Burwell21 (F1) BuzzLyseyear (F1) ByChance (F1) Byougenkin (F1) Cabrinians (F1) CaptainTrips (F1) Cerasum (F1) Che8 (F1) Chevrolet (F1) ChickenDinner (F1) Chuckly (F1) Clifton (F1) Coco12 (F1) Cornucopia (F1) Crumble (F1) DaddyRickover (F1) Daenerys (F1) Dante (F1) DaWorst (F1) DeadP (F1) Deb65 (F1) DillTech15 (F1) DirtMcgirt (F1) DLane (F1) DocMcStuffins (F1) Donkeykong (F1) Doomphist (F1) Dorothy (F1) DosHalletts (F1) DotProduct (F1) Doug (F1) Drago (F1) DRBy19 (F1) Eish (F1) EleanorGeorge (F1) Emma (F1) Empress (F1) Enby (F1) Eradicator (F1) Estave1 (F1) Fancypants (F1) Fastidio (F1) Filuzino (F1) Firehouse51 (F1) Flathead (F1) Florinda (F1) Frankie (F1) FreddyB (F1) Fruitloop (F1) Galactic (F1) Gandalph (F1) Geralt (F1) GigiOuiOui (F1) Girafales (F1) Girr (F1) Gorge (F1) GUmbie (F1) Guppsters (F1) Hades (F1) Hamulus (F1) Harley (F1) Hegedechwinu (F1) Hlubikazi (F1) Ibhubesi (F1) IbOuu (F1) IMpilo (F1) Inventum (F1) IrishSherpFalk (F1) JalFarm20 (F1) James (F1) Jarcob (F1) Jinglebell (F1) Job42 (F1) JoeyJr (F1) Juice456 (F1) Juniper1 (F1) Karhdo (F1) Kenuha5 (F1) Kersh (F1) Kimberlium (F1) KingMidas (F1) Kingsley (F1) Koella (F1) Krakatau (F1) KristaRAM (F1) LastJedi (F1) Latretium (F1) Leozinho (F1) Leperchaun (F1) LilMoolah (F1) LittleShirley (F1) Lizziana (F1) Llama (F1) Llij (F1) Lorde (F1) LunaBlu (F1) Madiba (F1) MadMen (F1) Mahavrat (F1) Mandlovu (F1) Mantra (F1) Maravista (F1) Marker (F1) Martik (F1) Mattes (F1) Melissauren88 (F1) MilanaBonita (F1) MilleniumForce (F1) MinionDave (F1) Minnie (F1) Misha28 (F1) MisterCuddles (F1) Modragons (F1) Moonbeam (F1) Mova (F1) Mozy (F1) Ms6 (F1) MulchExplorer (F1) Mutaforma13 (F1) Nimbo (F1) Nitzel (F1) Nivrat (F1) NormanBulbieJr (F1) Ochi17 (F1) OfUltron (F1) Ogopogo (F1) Oksu (F1) OldBen (F1) OlympiaSaint (F1) Ovechkin (F1) OwlsT2W (F1) Pacc40 (F1) Phalconet (F1) Phanphagia (F1) PHappiness (F1) Phasih (F1) Phatniss (F1) PhesterPhotato (F1) Philus (F1) Piper2020 (F1) Pippy (F1) Plumbus (F1) PMC (F1) Poenanya (F1) Polka14 (F1) Pollywog (F1) PopTart (F1) Priscilla (F1) QuickMath (F1) Quico (F1) Radiance (F1) Ramsey (F1) RedBird (F1) Rialto (F1) Rita (F1) RitaG (F1) Rockne (F1) RockyHorror (F1) Royals2015 (F1) Ruby (F1) Saal (F1) Sabbb (F1) Sandalphon (F1) Sarma624 (F1) Sassafras (F1) SassyB (F1) Scottish (F1) Seabastian (F1) Seagreen (F1) SG4 (F1) Shauna1 (F1) ShiLan (F1) ShowerHandel (F1) ShroomBoi (F1) SimranZ1 (F1) SiSi (F1) Sparkdehlily (F1) Spartacus (F1) Spikelee (F1) Spoonbill (F1) StAnnes (F1) Stap (F1) Starcevich (F1) Strokeseat (F1) SuperGrey (F1) SwagPigglett (F1) Taj (F1) TDanisky (F1) TootsiePop (F1) Tootsieroll (F1) Totinger (F1) Tweety (F1) UncleRicky (F1) Velveteen (F1) Veteran (F1) VRedHorse (F1) Wachhund (F1) Wee (F1) Whatsapiecost (F1) Whouxphf (F1) WillSterrel (F1) XFactor (F1) Yorick (F1) Zerg (F1) Zizzle (F1) Avani (F2) Che9d (F2) Demsculpinboyz (F2) Jabbawokkie (F2) Soul22 (F2) Yoshi (F2) Zapner (F2) Squirty (F3) LunaStella (F4) Renaud18 (F4) TChen (F4) ThetaBob (F4) Cornie (F5) Toron (F6)
Annotating Institution	University of California, Los Angeles
Annotation Status	In GenBank
Morphotype	Siphoviridae
Number of Genes	104
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View DRBy19_1 (terminase small subunit) DRBy19_2 (terminase large subunit) DRBy19_3 (portal protein) DRBy19_4 (capsid maturation protease and MuF-like fusion protein) DRBy19_5 (scaffolding protein) DRBy19_6 (major capsid protein) DRBy19_7 (head-to-tail adaptor) DRBy19_8 (head-to-tail stopper) DRBy19_9 DRBy19_10 (tail terminator) DRBy19_11 (major tail protein) DRBy19_12 (tail assembly chaperone) DRBy19_13 (tail assembly chaperone) DRBy19_14 (tape measure protein) DRBy19_15 (minor tail protein) DRBy19_16 (minor tail protein) DRBy19_17 (minor tail protein) DRBy19_18 (minor tail protein) DRBy19_19 (minor tail protein) DRBy19_20 (minor tail protein) DRBy19_21 (minor tail protein) DRBy19_22 DRBy19_23 (minor tail protein) DRBy19_24 (minor tail protein) DRBy19_25 (helix-turn-helix DNA-binding domain protein) DRBy19_26 DRBy19_27 DRBy19_28 DRBy19_29 DRBy19_30 (lysin A) DRBy19_31 (lysin B) DRBy19_32 (holin) DRBy19_33 DRBy19_34 DRBy19_35 (DnaQ-like DNA polymerase III subunit) DRBy19_36 DRBy19_37 DRBy19_38 DRBy19_39 DRBy19_40 DRBy19_41 DRBy19_42 DRBy19_43 (tyrosine integrase) DRBy19_44 (RelE-like toxin) DRBy19_45 (RelB-like antitoxin) DRBy19_46 DRBy19_47 (immunity repressor) DRBy19_48 (Cro protein) DRBy19_49 (antirepressor) DRBy19_50 DRBy19_51 DRBy19_52 DRBy19_53 DRBy19_54 (excise) DRBy19_55 DRBy19_56 DRBy19_57 DRBy19_58 DRBy19_59 DRBy19_60 (WhiB family transcription factor) DRBy19_61 (helix-turn-helix DNA-binding domain protein) DRBy19_62 (WhiB family transcription factor) DRBy19_63 (helix-turn-helix DNA-binding domain protein) DRBy19_64 DRBy19_65 DRBy19_66 DRBy19_67 (helix-turn-helix DNA-binding domain protein) DRBy19_68 (RecA-like DNA recombinase) DRBy19_69 (HNH endonuclease) DRBy19_70 (helix-turn-helix DNA-binding domain protein) DRBy19_71 DRBy19_72 (DNA methylase) DRBy19_73 DRBy19_74 DRBy19_75 DRBy19_76 DRBy19_77 (G-I-Y Y-I-G endonuclease) DRBy19_78 (helix-turn-helix DNA-binding domain protein) DRBy19_79 DRBy19_80 DRBy19_81 DRBy19_82 DRBy19_83 DRBy19_84 DRBy19_85 DRBy19_86 DRBy19_87 (MPME1 protein) DRBy19_88 DRBy19_89 DRBy19_90 DRBy19_91 DRBy19_92 DRBy19_93 DRBy19_94 (G-I-Y Y-I-G endonuclease) DRBy19_95 DRBy19_96 DRBy19_97 DRBy19_98 DRBy19_99 DRBy19_100 DRBy19_101 (glycosyltransferase) DRBy19_102 DRBy19_103 (glycosyltransferase) DRBy19_104 (HNH endonuclease)
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MT310893
Refseq Number	None yet
Archiving Info
Archiving status	Archived
SEA Lysate Titer	4e6
Pitt Freezer Box#	27
Pitt Freezer Box Grid#	C8
Available Files
GenBank File for Phamerator	Download

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