Mycobacterium phage Deb65
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Detailed Information for Phage Deb65
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesSweetTart
Found ByGianna Dunn
Year Found2023
Location FoundWilliamsburg, VA USA
Finding InstitutionCollege of William & Mary
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates37.27085 N, 76.71576 W Map
Discovery NotesThe soil that I collected my sample from was found in a moist environment in a swamp-like area of the trails.
Naming NotesI named my phage after sweet tart rope candy because the tail of my phage bacteria is long like the candy.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 23, 2024
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1119
Genome length (bp)55767
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGACGGCGC
GC Content61.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Blarby (None)

MulchSalad (None)

Akhila (F1)

Alexphander (F1)

AlpineSix (F1)

ArcusAngelus (F1)

Ardmore (F1)

Aubs (F1)

Awesomesauce (F1)

Batiatus (F1)

Beakin (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BlueCrab (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chevrolet (F1)

ChickenDinner (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Crumble (F1)

DaddyRickover (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

Deb65 (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

DocMcStuffins (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DosHalletts (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Eradicator (F1)

Estave1 (F1)

Fancypants (F1)

Fastidio (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

FreddyB (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

GigiOuiOui (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Guppsters (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

IbOuu (F1)

IMpilo (F1)

Inventum (F1)

IrishSherpFalk (F1)

JalFarm20 (F1)

James (F1)

Jarcob (F1)

Jinglebell (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Juniper1 (F1)

Karhdo (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

Leozinho (F1)

Leperchaun (F1)

LilMoolah (F1)

LittleShirley (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

LunaBlu (F1)

Madiba (F1)

MadMen (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Maravista (F1)

Marker (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilanaBonita (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Ms6 (F1)

MulchExplorer (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

Oksu (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phalconet (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

PhesterPhotato (F1)

Philus (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Radiance (F1)

Ramsey (F1)

RedBird (F1)

Rialto (F1)

Rita (F1)

RitaG (F1)

Rockne (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sabbb (F1)

Sandalphon (F1)

Sarma624 (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

ShowerHandel (F1)

ShroomBoi (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Stap (F1)

Starcevich (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tootsieroll (F1)

Totinger (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

VRedHorse (F1)

Wachhund (F1)

Wee (F1)

Whatsapiecost (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Yorick (F1)

Zerg (F1)

Zizzle (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Soul22 (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

LunaStella (F4)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Toron (F6)

Annotating InstitutionCollege of William & Mary
Annotation StatusIn GenBank
Plaque NotesMy first enrichment plating cleared and then after that I had webbed lysis and lots of plaque.
MorphotypeSiphoviridae
Number of Genes97
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Deb65_1
(terminase, small subunit)

Deb65_2
(terminase, large subunit)

Deb65_3
(portal protein)

Deb65_4
(capsid maturation protease)

Deb65_5
(scaffolding protein)

Deb65_6
(major capsid protein)

Deb65_7
(head-to-tail adaptor)

Deb65_8
(head-to-tail stopper)

Deb65_9

Deb65_10
(tail terminator)

Deb65_11
(major tail protein)

Deb65_12
(tail assembly chaperone)

Deb65_13
(tail assembly chaperone)

Deb65_14
(tape measure protein)

Deb65_15
(minor tail protein)

Deb65_16
(minor tail protein)

Deb65_17
(minor tail protein)

Deb65_18
(minor tail protein)

Deb65_19
(minor tail protein)

Deb65_20
(minor tail protein)

Deb65_21

Deb65_22

Deb65_23

Deb65_24
(minor tail protein)

Deb65_25
(helix-turn-helix DNA binding domain)

Deb65_26

Deb65_27

Deb65_28

Deb65_29

Deb65_30
(lysin A)

Deb65_31
(lysin B)

Deb65_32
(holin)

Deb65_33

Deb65_34

Deb65_35
(DnaQ-like DNA polymerase III subunit)

Deb65_36

Deb65_37

Deb65_38

Deb65_39

Deb65_40
(excise)

Deb65_41
(tyrosine integrase)

Deb65_42

Deb65_43
(immunity repressor)

Deb65_44
(Cro (control of repressor's operator))

Deb65_45

Deb65_46

Deb65_47

Deb65_48

Deb65_49

Deb65_50

Deb65_51

Deb65_52
(WhiB family transcription factor)

Deb65_53

Deb65_54

Deb65_55

Deb65_56

Deb65_57

Deb65_58
(RecA-like DNA recombinase)

Deb65_59

Deb65_60
(HNH endonuclease)

Deb65_61

Deb65_62

Deb65_63
(DnaQ-like DNA polymerase III subunit)

Deb65_64
(DNA methyltransferase)

Deb65_65

Deb65_66

Deb65_67
(DNA methyltransferase)

Deb65_68

Deb65_69

Deb65_70

Deb65_71

Deb65_72

Deb65_73

Deb65_74

Deb65_75

Deb65_76

Deb65_77

Deb65_78

Deb65_79

Deb65_80

Deb65_81

Deb65_82

Deb65_83

Deb65_84

Deb65_85

Deb65_86

Deb65_87
(helix-turn-helix DNA binding domain)

Deb65_88

Deb65_89

Deb65_90

Deb65_91

Deb65_92

Deb65_93

Deb65_94
(glycosyltransferase)

Deb65_95

Deb65_96
(glycosyltransferase)

Deb65_97
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPQ184836
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 186
Pitt Freezer Box Grid# B8
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload