| Detailed Information for Phage Deb65 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | SweetTart |
| Found By | Gianna Dunn |
| Year Found | 2023 |
| Location Found | Williamsburg, VA USA |
| Finding Institution | College of William & Mary |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 37.27085 N, 76.71576 W Map |
| Discovery Notes | The soil that I collected my sample from was found in a moist environment in a swamp-like area of the trails. |
| Naming Notes | I named my phage after sweet tart rope candy because the tail of my phage bacteria is long like the candy. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1119 |
| Genome length (bp) | 55767 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGACGGCGC |
| GC Content | 61.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | College of William & Mary |
| Annotation Status | In GenBank |
| Plaque Notes | My first enrichment plating cleared and then after that I had webbed lysis and lots of plaque. |
| Morphotype | Siphoviridae |
| Number of Genes | 97 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ184836 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
186 |
| Pitt Freezer Box Grid# |
B8 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
