| Detailed Information for Phage DirtMcgirt |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | John Brooker |
| Year Found | 2012 |
| Location Found | Charleston, SC USA |
| Finding Institution | College of Charleston |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 32.782927 N, 79.945389 W Map |
| Discovery Notes | Found in muddy driveway by puddles of recent flood water almost black in color |
| Naming Notes | To denote contamination of a set of streaks I labeled them as "Dirt Mcgirt". Upon seeing the label, my instructor noted that she liked my phage name. And so it became my phage name. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 31, 2013 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 |
| Sequencer Used | 454 Genome Sequencer FLX |
| Approximate Shotgun Coverage | 260 |
| Sanger Finishing Reactions | 2 |
| Genome length (bp) | 54396 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CCGATGGCAT |
| GC Content | 61.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Rockland Community College |
| Annotation Status | In GenBank |
| Plaque Notes | Lytic, 0.5cm plaque diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 104 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ681512 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
34 |
| Pitt Freezer Box Grid# |
47 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
