| Detailed Information for Phage Eish |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Ian Carleton |
| Year Found | 2018 |
| Location Found | Empangeni, South Africa |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 28.8 S, 31.8 E Map |
| Discovery Notes | Discovered from a lysate of phage "Thonko" collected from Empangeni, South Africa by Nomusa Christina Mzimela. The lysate was plaque purified at the University of Pittsburgh, and this phage was discovered after plaque purification demonstrated two distinct plaque morphologies. |
| Naming Notes | Colloquial saying common in South African culture.
A South African word derived from Xhosa, now used in all languages within South Africa and some neighbouring countries. Eish is one of the reasons South Africa is so great, it can express anything from excitement to horror. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 19, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 650 |
| Genome length (bp) | 58150 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGACGGCGC |
| GC Content | 61.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Number of Genes | 109 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN585975 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Ignore |
| Available Files |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |