| Detailed Information for Phage Gandalph |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Emily Carlson |
| Year Found | 2011 |
| Location Found | Spokane, WA USA |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 47.667944 N, 117.404394 W Map |
| Discovery Notes | Gandalph was captured on September 2nd 2011, in a moist flowerbed, underneath a pink rosebush behind St Aloysius Church in Spokane, WA. The three-tablespoon sample of soil was collected at 2 inches and the temperature was 69°F. The soil itself was chunky and moist. The environment was at an elevation of 1913ft. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 28, 2019 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 9042 |
| Genome length (bp) | 54974 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGACGGCGC |
| GC Content | 61.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Gonzaga University |
| Annotation Status | In GenBank |
| Plaque Notes | An image of the 10^-6 dilution of the HTL of Gandalph showing the plaque morphology. The plaques are small with clear edges. The white swirls are colonies of M. smegmatis. |
| Morphotype | Siphoviridae |
| Number of Genes | 104 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT684586 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2011GONZgandalphEAC |
| Pitt Freezer Box# |
24 |
| Pitt Freezer Box Grid# |
17 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
