| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Harsha Jujjavarapu and Aakash Gandhi |
| Year Found | 2012 |
| Location Found | St. Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.6457 N, 90.3114 W Map |
| Discovery Notes | Collected on 9/2/2012 at 4:37 pm.
Temperature was 84 F and sample was at a depth of around 6 inches.
This sample was taken from the Burning Kumquat which is a student-run organic garden located on Shepley Drive. This carrot patch only just geminated into seedlings and is regularly fertilized with compost.
Sample site was teeming with life (ants, aphids). |
| Naming Notes | The name of this phage came from the robot from the TV show Invader Zim. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jun 4, 2013 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | 454 |
| Approximate Shotgun Coverage | 397 |
| Sanger Finishing Reactions | 7 |
| Genome length (bp) | 57754 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGACGGCGC |
| GC Content | 61.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of South Florida |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were medium sized and clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 102 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH669003 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.28*10^10 |
| Pitt Freezer Box# |
42 |
| Pitt Freezer Box Grid# |
13 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
