| Detailed Information for Phage Grimmer |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Bleh |
| Found By | Jammie Holland and Shaker Algahthani |
| Year Found | 2018 |
| Location Found | Butte, MT United States |
| Finding Institution | Montana Technological University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 37°C |
| GPS Coordinates | 46.0038 N, 112.5348 W Map |
| Discovery Notes | Discovered by Jammie Holland and Shaker Algathani in 2018. Isolated as "Bleh" by Shea Earley in 2023. Further analysis, purification, PCR, and DNA extraction performed by Dawson Roesti in 2024. |
| Naming Notes | Phage Grimmer is named after the character Wolfgang Grimmer (aka The Magnificent Steiner) in the popular anime and manga titled "Monster". |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 8, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 7243 |
| Genome length (bp) | 55294 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGAAGGCGC |
| GC Content | 61.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | F Cluster phages are generally temperate. Lysogen testing results suggest that phage Grimmer forms lysogenic bacteria. |
| Other Cluster Members |
|
| Annotating Institution | Montana Technological University |
| Annotation Status | Being Annotated (Expected completion by 12/15/2025) |
| Plaque Notes | Plaque morphologies are larger in size with a cloudy halo surrounding a clear center. |
| Morphotype | Siphoviridae |
| Number of Genes | 111 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
203 |
| Pitt Freezer Box Grid# |
G10 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
