| Detailed Information for Phage Harley |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Leanne Pillay |
| Year Found | 2015 |
| Location Found | Durban, South Africa |
| Finding Institution | University of Kwazulu-Natal |
| Program | Mycobacterial Genetics Course, Durban, South Africa |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 29.926212 S, 30.895681 E Map |
| Discovery Notes | The soil was taken from my garden at home, from around the rose bushes. |
| Naming Notes | This phage is named after my pitbull Harley who passed away earlier this year. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 27, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2848 |
| Genome length (bp) | 58731 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGAAGGCGC |
| GC Content | 61.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Kwazulu-Natal |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques presented themselves as distinct, circular zones of clearing, with clear centers and measured approximately 1.8mm in diameter |
| Morphotype | Siphoviridae |
| Number of Genes | 107 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH632119 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |