| Detailed Information for Phage Inventum |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Matthew Karssen |
| Year Found | 2012 |
| Location Found | Holland, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 42.787304 N, 86.102476 W Map |
| Discovery Notes | Isolated from enriched soil sample. The soil was taken from about 4 cm down, approximately two feet from a high traffic sidewalk on Hope College's campus. The soil was slightly sandy and damp, and was under the shade of a moderate sized bush. There was no grass cover, but a minor amount of decomposing matter. |
| Naming Notes | Inventum (Pronounced: een-wen-toom) means "Discovery" in Latin. I've always loved the Latin language, so I decided to name my phage in Latin. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 11, 2013 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent |
| Approximate Shotgun Coverage | 600 |
| Sanger Finishing Reactions | 6 |
| Genome length (bp) | 57052 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGACGGCGC |
| GC Content | 61.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were oblong, measuring approximately 2.5 x 3.5 mm at 24 hours, and about 3.5 x 4.5 mm at 48 hours. The plaques showed two distinct areas of turbidity. The inner 1 - 1.5 mm (diameter) was visibly less turbid than the outer area. Plaque size was very density independent, only changing slightly with differences in number of plaques on the plate. |
| Morphotype | Siphoviridae |
| Number of Genes | 102 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KM066034 |
| Refseq Number | NC_026596 |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
36 |
| Pitt Freezer Box Grid# |
49 |
| Available Files |
| Restriction Digest Picture | Download |
| EM Picture | Download |
