| Detailed Information for Phage James |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Garrett Santini |
| Year Found | 2015 |
| Location Found | Wayne, NJ, NJ USA |
| Finding Institution | Lehigh University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | -3°C |
| GPS Coordinates | 40.910833 N, 74.261667 W Map |
| Naming Notes | During the processes of phage titer assays, this phage had acquired the nickname "The Great Plaquesby" which was inspired by F. Scott Fitzgerald's "The Great Gatsby." Based off of this nickname, the name James was chosen to represent James Gatsby from the novel. Since James is such an exciting phage, it couldn't be more fitting that his name comes out of a novel written about the roaring 1920s in America. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 5, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1667 |
| Genome length (bp) | 59617 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGACGGCGC |
| GC Content | 61.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Lehigh University |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 108 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK524517 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2x10^9 |
| Pitt Freezer Box# |
15 |
| Pitt Freezer Box Grid# |
G7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
