| Detailed Information for Phage Juniper1 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Berklium |
| Found By | Berkley Walton |
| Year Found | 2022 |
| Location Found | Bowling Green, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 24°C |
| GPS Coordinates | 36.978766 N, 86.466343 W Map |
| Discovery Notes | Soil sample was collected from a compost pot. Phage contents were abundant producing completely clear plates. Approximately five serial dilutions were performed, not because of a failed 10-fold dilution or contamination, but because of having such high concentration. A ten-fold dilution was accomplished each round and each weeks results were consistent with proper techniques. |
| Naming Notes | A series of names were shuffles through when finally, my cat's name Juniper with the number 1 applied to end was processed by the database. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 11, 2023 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 3786 |
| Genome length (bp) | 57275 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGAAGGCGC |
| GC Content | 61.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | Phage contents were abundant throughout multiple serial dilutions producing completely clear plates. After four serial dilutions, individual plaques began to form instead of plaque clusters. Plaques sizes averaged approximately 0.3cm. |
| Morphotype | Siphoviridae |
| Number of Genes | 99 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ201084 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.5X10^11 |
| Pitt Freezer Box# |
157 |
| Pitt Freezer Box Grid# |
G2 |
| Available Files |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
