| Detailed Information for Phage Kenuha5 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Nur Ashykeen Mohd Fauzi |
| Year Found | 2017 |
| Location Found | Boulder, CO USA |
| Finding Institution | University of Colorado Boulder |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.012583 N, 105.269167 W Map |
| Discovery Notes | The soil sample was quite damp and collected somewhere at bushes near the Rec. Center of the University of Colorado Boulder. |
| Naming Notes | The name came from the combination of Nur's nickname "Ken" with her lab partner's "Nuha". The number 5 reflects that the phage was found at the fifth trial of bacteriophage enrichment. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 1, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 296 |
| Genome length (bp) | 56502 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGAAGGCGC |
| GC Content | 61.7% |
| Sequencing Notes | This phage was sequenced as part of a mixed DOGEMS sample. The sequence of this genome was confirmed to belong to this phage by PCR. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Colorado Boulder |
| Annotation Status | In GenBank |
| Plaque Notes | Clear spots with slight turbid around the edges
Approximately 1-2nm
48 hours at 37°C |
| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN369739 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
61 |
| Pitt Freezer Box Grid# |
G7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
