| Detailed Information for Phage Kersh |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Joseph Chang |
| Year Found | 2014 |
| Location Found | La Jolla, CA USA |
| Finding Institution | University of California, San Diego |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 32.876853 N, 117.239147 W Map |
| Discovery Notes | From compost heap of decomposing eucalyptus leaves. Humid weather, compost heap damp. Collected from underneath top layer of soil. |
| Naming Notes | Named after Clayton Kershaw, Los Angeles Dodgers pitcher. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 12, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1113 |
| Genome length (bp) | 60190 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTAGGCGC |
| GC Content | 61.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | After initial enrichment, the plaque was about 3-4.5 mm in diameter with a turbid outer ring. During early stages of purification however, the phage was only 1-2 mm in diameter with no signs of lysogeny and was extremely clear. Plaque morphology returned to early stages of enrichment during lysates and harvesting. |
| Morphotype | Siphoviridae |
| Number of Genes | 107 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX610764 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
