| Detailed Information for Phage Kingsley |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Steven Black |
| Year Found | 2015 |
| Location Found | Blacksburg, VA USA |
| Finding Institution | Virginia Tech |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 37.24731 N, 80.4175 W Map |
| Discovery Notes | From Potted Plant (genus Schlumbergera)
Plant had sat outside for a month, then was moved inside for over a month before the sample was taken. |
| Naming Notes | I just thought it sounded good |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 10, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1576 |
| Genome length (bp) | 60300 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CCGATGGCAT |
| GC Content | 61.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | varying sizes of plaques, but all are very small and clear |
| Morphotype | Siphoviridae |
| Number of Genes | 112 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KY702574 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.385E8 |
| Date of SEA Lysate Titering |
Oct 13, 2015 |
| Pitt Freezer Box# |
20 |
| Pitt Freezer Box Grid# |
H8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
