| Detailed Information for Phage LastJedi |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Duplicates of this Phage | MargotMini |
| Found By | Jewel Joseph and Don Kiriella |
| Year Found | 2018 |
| Location Found | Old Westbury, NY USA |
| Finding Institution | SUNY Old Westbury |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 75°C |
| GPS Coordinates | 40.798447 N, 73.57308 W Map |
| Discovery Notes | Shrubby Area in-between Natural Science Building and Campus Center at SUNY Old Westbury. It had been raining for a few days before and the ground was muddy. It was near a bush. |
| Naming Notes | We named our phage LastJedi because we are Star Wars fans. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 31, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1652 |
| Genome length (bp) | 55149 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGACGGCGC |
| GC Content | 61.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | College of Charleston |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques is close to circular but not perfect, clear plaques with a titer of 5.2 * 10^10 PFU/ml, with a DNA concentration 90 nanograms per microliter. |
| Morphotype | Siphoviridae |
| Number of Genes | 94 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT553345 |
| Refseq Number | None yet |
| Published in Paper | Davis et al 2020, Microbiology Resource Announcements: Genome Sequences of the Mycobacteriophages Awesomesauce and LastJedi |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
88 |
| Pitt Freezer Box Grid# |
H5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
