| Detailed Information for Phage Mahavrat |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Deeya Patel |
| Year Found | 2016 |
| Location Found | Hopkinsville, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 72°C |
| GPS Coordinates | 36.9855 N, 86.4492 W Map |
| Discovery Notes | Phage Mahavrat was isolated on 9/27/16 from moist dark brown soil rich with decaying plant matter, at a depth of 12 cm. The soil was taken near a greenhouse next to the Engineering and Biological Sciences Building at Western Kentucky University. The temperature was 76F at time of collection. Mahavrat was isolated using an enrichment culture incubated at 30C. |
| Naming Notes | This phage is named after my grandfather, Mahavrat, the biggest influencer on my education. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 3, 2017 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 268 |
| Genome length (bp) | 55945 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGAAGGCGC |
| GC Content | 61.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were clear, lytic and circular in their morphology. |
| Morphotype | Siphoviridae |
| Number of Genes | 99 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK967397 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2016WEKUmahavratDP |
| SEA Lysate Titer |
3.8 x 10^12 pfu/mL |
| Date of SEA Lysate Titering |
Nov 15, 2016 |
| Pitt Freezer Box# |
37 |
| Pitt Freezer Box Grid# |
C10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
