Mycobacterium phage MilanaBonita
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Detailed Information for Phage MilanaBonita
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByRita Reddy
Year Found2018
Location FoundCentereach, NY United States
Finding InstitutionSUNY Old Westbury
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates40.86299 N, 73.10535 W Map
Discovery NotesSoil sample was taking from a flower bed and the soil was treated with horse manure.
Naming NotesMilanaBonita is the name of the bird the movie character Azarias raises from when it was a chick in the movie Los Santos Inocentes, based on the book with the same title from Miguel Delibes.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJul 8, 2020
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage176
Genome length (bp)56251
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGAAGGCGC
GC Content62.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterF
SubclusterF1
Cluster Life CycleTemperate
Other Cluster Members

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Blarby (None)

MulchSalad (None)

Akhila (F1)

Alexphander (F1)

AlpineSix (F1)

ArcusAngelus (F1)

Ardmore (F1)

Aubs (F1)

Awesomesauce (F1)

Batiatus (F1)

Beakin (F1)

BigPhil (F1)

Bipolar (F1)

Blexus (F1)

BlueCrab (F1)

BobaPhett (F1)

Bobi (F1)

BodEinwohner17 (F1)

Boomer (F1)

Brocalys (F1)

Bubbles123 (F1)

Burwell21 (F1)

BuzzLyseyear (F1)

ByChance (F1)

Byougenkin (F1)

Cabrinians (F1)

CaptainTrips (F1)

Cerasum (F1)

Che8 (F1)

Chevrolet (F1)

ChickenDinner (F1)

Chuckly (F1)

Clifton (F1)

Coco12 (F1)

Cornucopia (F1)

Crumble (F1)

DaddyRickover (F1)

Daenerys (F1)

Dante (F1)

DaWorst (F1)

DeadP (F1)

Deb65 (F1)

DillTech15 (F1)

DirtMcgirt (F1)

DLane (F1)

DocMcStuffins (F1)

Donkeykong (F1)

Doomphist (F1)

Dorothy (F1)

DosHalletts (F1)

DotProduct (F1)

Doug (F1)

Drago (F1)

DRBy19 (F1)

Eish (F1)

EleanorGeorge (F1)

Emma (F1)

Empress (F1)

Enby (F1)

Eradicator (F1)

Estave1 (F1)

Fancypants (F1)

Fastidio (F1)

Filuzino (F1)

Firehouse51 (F1)

Flathead (F1)

Florinda (F1)

Frankie (F1)

FreddyB (F1)

Fruitloop (F1)

Galactic (F1)

Gandalph (F1)

Geralt (F1)

GigiOuiOui (F1)

Girafales (F1)

Girr (F1)

Gorge (F1)

GUmbie (F1)

Guppsters (F1)

Hades (F1)

Hamulus (F1)

Harley (F1)

Hegedechwinu (F1)

Hlubikazi (F1)

Ibhubesi (F1)

IbOuu (F1)

IMpilo (F1)

Inventum (F1)

IrishSherpFalk (F1)

JalFarm20 (F1)

James (F1)

Jarcob (F1)

Jinglebell (F1)

Job42 (F1)

JoeyJr (F1)

Juice456 (F1)

Juniper1 (F1)

Karhdo (F1)

Kenuha5 (F1)

Kersh (F1)

Kimberlium (F1)

KingMidas (F1)

Kingsley (F1)

Koella (F1)

Krakatau (F1)

KristaRAM (F1)

LastJedi (F1)

Latretium (F1)

Leozinho (F1)

Leperchaun (F1)

LilMoolah (F1)

LittleShirley (F1)

Lizziana (F1)

Llama (F1)

Llij (F1)

Lorde (F1)

LunaBlu (F1)

Madiba (F1)

MadMen (F1)

Mahavrat (F1)

Mandlovu (F1)

Mantra (F1)

Maravista (F1)

Marker (F1)

Martik (F1)

Mattes (F1)

Melissauren88 (F1)

MilanaBonita (F1)

MilleniumForce (F1)

MinionDave (F1)

Minnie (F1)

Misha28 (F1)

MisterCuddles (F1)

Modragons (F1)

Moonbeam (F1)

Mova (F1)

Mozy (F1)

Ms6 (F1)

MulchExplorer (F1)

Mutaforma13 (F1)

Nimbo (F1)

Nitzel (F1)

Nivrat (F1)

NormanBulbieJr (F1)

Ochi17 (F1)

OfUltron (F1)

Ogopogo (F1)

Oksu (F1)

OldBen (F1)

OlympiaSaint (F1)

Ovechkin (F1)

OwlsT2W (F1)

Pacc40 (F1)

Phalconet (F1)

Phanphagia (F1)

PHappiness (F1)

Phasih (F1)

Phatniss (F1)

PhesterPhotato (F1)

Philus (F1)

Piper2020 (F1)

Pippy (F1)

Plumbus (F1)

PMC (F1)

Poenanya (F1)

Polka14 (F1)

Pollywog (F1)

PopTart (F1)

Priscilla (F1)

QuickMath (F1)

Quico (F1)

Radiance (F1)

Ramsey (F1)

RedBird (F1)

Rialto (F1)

Rita (F1)

RitaG (F1)

Rockne (F1)

RockyHorror (F1)

Royals2015 (F1)

Ruby (F1)

Saal (F1)

Sabbb (F1)

Sandalphon (F1)

Sarma624 (F1)

Sassafras (F1)

SassyB (F1)

Scottish (F1)

Seabastian (F1)

Seagreen (F1)

SG4 (F1)

Shauna1 (F1)

ShiLan (F1)

ShowerHandel (F1)

ShroomBoi (F1)

SimranZ1 (F1)

SiSi (F1)

Sparkdehlily (F1)

Spartacus (F1)

Spikelee (F1)

Spoonbill (F1)

StAnnes (F1)

Stap (F1)

Starcevich (F1)

Strokeseat (F1)

SuperGrey (F1)

SwagPigglett (F1)

Taj (F1)

TDanisky (F1)

TootsiePop (F1)

Tootsieroll (F1)

Totinger (F1)

Tweety (F1)

UncleRicky (F1)

Velveteen (F1)

Veteran (F1)

VRedHorse (F1)

Wachhund (F1)

Wee (F1)

Whatsapiecost (F1)

Whouxphf (F1)

WillSterrel (F1)

XFactor (F1)

Yorick (F1)

Zerg (F1)

Zizzle (F1)

Avani (F2)

Che9d (F2)

Demsculpinboyz (F2)

Jabbawokkie (F2)

Soul22 (F2)

Yoshi (F2)

Zapner (F2)

Squirty (F3)

LunaStella (F4)

Renaud18 (F4)

TChen (F4)

ThetaBob (F4)

Cornie (F5)

Toron (F6)

Annotating InstitutionSUNY Old Westbury
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Number of Genes99
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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MilanaBonita_1
(HNH endonuclease)

MilanaBonita_2
(terminase small subunit)

MilanaBonita_3
(terminase large subunit)

MilanaBonita_4
(portal protein)

MilanaBonita_5
(capsid maturation protease)

MilanaBonita_6
(scaffolding protein)

MilanaBonita_7
(major capsid protein)

MilanaBonita_8
(head-to-tail adaptor)

MilanaBonita_9
(head-to-tail stopper)

MilanaBonita_10

MilanaBonita_11
(tail terminator)

MilanaBonita_12
(major tail protein)

MilanaBonita_13
(tail assembly chaperone)

MilanaBonita_14
(tail assembly chaperone)

MilanaBonita_15
(tape measure protein)

MilanaBonita_16
(minor tail protein)

MilanaBonita_17
(minor tail protein)

MilanaBonita_18
(minor tail protein)

MilanaBonita_19
(minor tail protein)

MilanaBonita_20
(minor tail protein)

MilanaBonita_21

MilanaBonita_22

MilanaBonita_23

MilanaBonita_24
(lysin A)

MilanaBonita_25
(lysin B)

MilanaBonita_26
(holin)

MilanaBonita_27

MilanaBonita_28

MilanaBonita_29
(DnaQ-like DNA polymerase III subunit)

MilanaBonita_30

MilanaBonita_31

MilanaBonita_32

MilanaBonita_33

MilanaBonita_34

MilanaBonita_35

MilanaBonita_36
(HNH endonuclease)

MilanaBonita_37
(tyrosine integrase)

MilanaBonita_38

MilanaBonita_39
(immunity repressor)

MilanaBonita_40
(Cro protein)

MilanaBonita_41

MilanaBonita_42
(excise)

MilanaBonita_99

MilanaBonita_43

MilanaBonita_44

MilanaBonita_45

MilanaBonita_46

MilanaBonita_47

MilanaBonita_48
(WhiB family transcription factor)

MilanaBonita_49

MilanaBonita_50
(helix-turn-helix DNA binding domain protein)

MilanaBonita_51

MilanaBonita_52

MilanaBonita_53

MilanaBonita_54

MilanaBonita_55
(HNH endonuclease)

MilanaBonita_56

MilanaBonita_57

MilanaBonita_58

MilanaBonita_59
(DNA methylase)

MilanaBonita_60
(ribbon-helix-helix DNA binding domain protein)

MilanaBonita_61

MilanaBonita_62

MilanaBonita_63

MilanaBonita_64

MilanaBonita_65

MilanaBonita_66
(DNA methylase)

MilanaBonita_67

MilanaBonita_68

MilanaBonita_69

MilanaBonita_70

MilanaBonita_71

MilanaBonita_72

MilanaBonita_73
(MPME1 protein)

MilanaBonita_74

MilanaBonita_75

MilanaBonita_76

MilanaBonita_77

MilanaBonita_78

MilanaBonita_79

MilanaBonita_80

MilanaBonita_81

MilanaBonita_82

MilanaBonita_83

MilanaBonita_84

MilanaBonita_85

MilanaBonita_86
(G-I-Y Y-I-G endonuclease)

MilanaBonita_87

MilanaBonita_88

MilanaBonita_89

MilanaBonita_90

MilanaBonita_91

MilanaBonita_92

MilanaBonita_93

MilanaBonita_94
(glycosyltransferase)

MilanaBonita_95
(serine/threonine kinase)

MilanaBonita_96

MilanaBonita_97
(glycosyltransferase)

MilanaBonita_98
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMZ681519
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 9x10^9 PFU/ml
Pitt Freezer Box# 128
Pitt Freezer Box Grid# C11
Available Files
GenBank File for PhameratorDownload