| Detailed Information for Phage Moonbeam |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Cassandra Michaels |
| Year Found | 2017 |
| Location Found | Butte, MT USA |
| Finding Institution | Helena High School |
| Program | Montana Tech Bringing Research Into the Classroom |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 46.5891 N, 112.0391 W Map |
| Discovery Notes | Discovered under the BRIC program at Montana Tech.
Genome annotation performed by Lauren Schmitt, Ozzie Ireland, and Abbie George.
SURF- amplification and DNA extraction by Hannah Sparks. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 3, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2558 |
| Genome length (bp) | 57984 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGAAGGCGC |
| GC Content | 61.1% |
| Sequencing Notes | The sequencing reads indicate that in ~60% of the population of phages from which DNA was extracted, there was a 587 bp deletion. It runs from 25659 to 26245 (inclusive). Despite the majority of the population being this shorter version, the final fasta contains the deleted portion since otherwise it would be lost. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Helena High School |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 107 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT684593 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
92 |
| Pitt Freezer Box Grid# |
A7 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
