| Detailed Information for Phage Mutaforma13 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Hannah Huntley |
| Year Found | 2010 |
| Location Found | Raleigh, NC USA |
| Finding Institution | North Carolina State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 35.782161 N, 78.666205 W Map |
| Discovery Notes | I collected my soil sample on the morning of August 24th, 2010. It was a cool morning, and it was lightly raining, therefore my soil sample was cool and moist. The air temperature was about 70 degrees.
The soil was under a thin layer of mulch, and I collected my sample from about an inch under the soil surface. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 22, 2011 |
| Genome length (bp) | 57701 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTAGGCGC |
| GC Content | 61.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | At high concentrations, my phage seems to exhibit characteristics
of a temperate life cycle. The plaques are small (1-2 mm in diameter) and turbid.
At lower concentrations, however, my phage seems to have a lytic life cycle. The
plaques are larger (3 mm in diameter) and are clear and round. They also have a
bulls-eye ring around them.
I think that the variation in plaque morphology may be due an ability of the phage
to communicate with others of its kind through chemical signaling. Maybe its life
cycle and replication rate are controlled by a protein that it secretes depending on
the concentration of itself in its environment. |
| Morphotype | Siphoviridae |
| Number of Genes | 105 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | JN020142 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2010NCSUmutaforma13HDH |
| SEA Lysate Titer |
1.6e11 |
| Date of SEA Lysate Titering |
Sep 28, 2010 |
| Pitt Freezer Box# |
15 |
| Pitt Freezer Box Grid# |
39 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
