| Detailed Information for Phage Nimbo |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Victoria Palochik |
| Year Found | 2017 |
| Location Found | Philadelphia, PA USA |
| Finding Institution | Drexel University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 26°C |
| GPS Coordinates | 39.96 N, 75.19 W Map |
| Discovery Notes | The soil sample was taken from one of the trees planted in the sidewalk outside of the WW Hagerty Library on Drexel University's campus.
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| Naming Notes | The phage was named after the cumulonimbus cloud. When the phage was on a plate with a low serial dilution, the plaques that would form would overlap. Like cumulonimbus clouds, I found you could make shapes and figures out of the overlapping, therefore sparking inspiration for the name. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 31, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1182 |
| Genome length (bp) | 55767 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGACGGCGC |
| GC Content | 61.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
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| Annotating Institution | Drexel University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques consisted of clear perfect circles approximately 0.30 mm in diameter with smooth margins. Also, the plaques has consistent morphology throughout the lab.
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| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
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| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH669009 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.2*10^11 pfu/mL |
| Pitt Freezer Box# |
60 |
| Pitt Freezer Box Grid# |
H7 |
| Available Files |
| GenBank File for Phamerator | Download |