| Detailed Information for Phage NormanBulbieJr |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Gillian Black and Vincent Zecchino |
| Year Found | 2017 |
| Location Found | Philadelphia, PA USA |
| Finding Institution | University of the Sciences (now St. Joseph's Univ) |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.9469 N, 75.2071 W Map |
| Discovery Notes | We discovered our phage in the soil outside of our university. The phage was dug up into a plastic bag and promptly isolated using direct isolation. |
| Naming Notes | This name was chosen to honor one of our lab teaching assistants and for our favorite tool in the phage lab, a bulbie. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 8, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1593 |
| Genome length (bp) | 58102 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGACGGCGC |
| GC Content | 61.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of the Sciences (now St. Joseph's Univ) |
| Annotation Status | In GenBank |
| Plaque Notes | The morphology of our phage is lytic. This means that it makes very clear plaques. Our phage tends to form plaques of multiple sizes. |
| Morphotype | Siphoviridae |
| Number of Genes | 102 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH399784 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
47 |
| Pitt Freezer Box Grid# |
D7 |
| Available Files |
| GenBank File for Phamerator | Download |