| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Alivia Colgan & Aubrey Neary |
| Year Found | 2023 |
| Location Found | New Oxford, PA United States |
| Finding Institution | Saint Joseph's University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 39.83976 N, 77.0523 W Map |
| Discovery Notes | Extremely rural location. No roads nearby and closest trees are approximately 12 meters away. Soil was collected from a garden bed. 80% humidity at time of collection. Sunny weather conditions. |
| Naming Notes | This soil sample was collected from the discoverer's father's garden. Specifically, the bed of soil that it was collected from previously grew potatoes. As a young child, her father always joked that if she were a boy, she would have been named "Fester." The name chosen combines these two ideas with the addition of "Ph" for phage. Therefore, the name of this phage is Phester Photato. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 12, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1551 |
| Genome length (bp) | 57622 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGACGGCGC |
| GC Content | 61.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Saint Joseph's University |
| Annotation Status | In GenBank |
| Plaque Notes | Most plaques have diameters of 7 mm while a few have diameters of 4 mm. Every plaque has three rings to form a distinct bullseye: a very clear center, a less clear middle ring, and a cloudy outer ring. The plaques are turbid rather than clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 109 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP978815 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.2 x 10^10 pfu/mL |
| Pitt Freezer Box# |
186 |
| Pitt Freezer Box Grid# |
H12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
