Mycobacterium phage Piper2020
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Discovery Information | |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Danielle Galli and Micaela Caron |
Year Found | 2016 |
Location Found | Melrose, MA USA |
Finding Institution | Merrimack College |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | No |
Isolation Temperature | Not entered |
GPS Coordinates | 42.451333 N, 71.076333 W Map |
Discovery Notes | The dirt sample that was tested was adopted from our Professor. It came from a tomato plant that was watered daily throughout the summer. |
Naming Notes | Our phage that we made in class out of different materials was named Piper. Micaela and I thought that this was an adorable name so we decided to name our discovered phage Piper. |
Sequencing Information | |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 10, 2017 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina |
Sequencer Used | Illumina MiSeq |
Read Type | Single-end reads |
Read Length | 150 bp |
Approximate Shotgun Coverage | 1883 |
Genome length (bp) | 57703 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CCGAAGGCAT |
GC Content | 62.7% |
Fasta file available? | Yes: Download fasta file |
Characterization | |
Cluster | F |
Subcluster | F1 |
Cluster Life Cycle | Temperate |
Other Cluster Members | |
Annotating Institution | Merrimack College |
Annotation Status | In GenBank |
Plaque Notes | The plaques that we obtained during this experiment were small and clear. |
Morphotype | Siphoviridae |
Number of Genes | 98 |
Number of tRNAs | 0 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List | |
Submitted Annotation in DNA Master Format | Download |
Submitted Minimal DNA Master File | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info | |
Uploaded to GenBank? | Yes |
GenBank Accession | MK494120 |
Refseq Number | None yet |
Archiving Info | |
Archiving status | Archived |
SEA Lysate Titer | 6.3*10^10 |
Pitt Freezer Box# | 73 |
Pitt Freezer Box Grid# | H2 |
Available Files | |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |