| Detailed Information for Phage Piper2020 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Danielle Galli and Micaela Caron |
| Year Found | 2016 |
| Location Found | Melrose, MA USA |
| Finding Institution | Merrimack College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.451333 N, 71.076333 W Map |
| Discovery Notes | The dirt sample that was tested was adopted from our Professor. It came from a tomato plant that was watered daily throughout the summer. |
| Naming Notes | Our phage that we made in class out of different materials was named Piper. Micaela and I thought that this was an adorable name so we decided to name our discovered phage Piper. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 10, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1883 |
| Genome length (bp) | 57703 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CCGAAGGCAT |
| GC Content | 62.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Merrimack College |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques that we obtained during this experiment were small and clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 98 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK494120 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
6.3*10^10 |
| Pitt Freezer Box# |
73 |
| Pitt Freezer Box Grid# |
H2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
