| Detailed Information for Phage Pollywog |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Grant Hughes |
| Year Found | 2017 |
| Location Found | Manhattan , KS USA |
| Finding Institution | Kansas State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 21°C |
| GPS Coordinates | 39.192467 N, 96.58427 W Map |
| Discovery Notes | found in September of 2017, roughly 4 inches deep in the dirt. There was a layer of mulch over the top of this dirt. was located right off of a main walking route through a college campus. The temperature that day was 65 to 70 degrees Fahrenheit. The sample was taken at 9:30 am. |
| Naming Notes | in Stranger Things 2 the baby Demogorgon is considered a pollywog. so yeah this is a baby Demogorgon virus user be cautious. Just kidding but that is where the name is from. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 12, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1658 |
| Genome length (bp) | 58397 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CCGATGGCAT |
| GC Content | 61.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Kansas State University |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Number of Genes | 105 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK359343 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
49 |
| Pitt Freezer Box Grid# |
A10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
