| Detailed Information for Phage Polo2Bam |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Nicole McGuire |
| Year Found | 2024 |
| Location Found | Colbert, AL United States |
| Finding Institution | Brigham Young University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 23°C |
| GPS Coordinates | 34.60813 N, 87.62755 W Map |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 10, 2025 |
| Sequencing Facility | CD Genomics |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NovaSeq X Plus |
| Read Type | Paired-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 6030 |
| Genome length (bp) | 57263 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGAAGGCGC |
| GC Content | 61.5% |
| Sequencing Notes | In the population of this phage that was sequenced, around half the reads indicated a small (~58 bp) deletion around 21930 bp. The final sequence reported does not have that deletion. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Brigham Young University |
| Annotation Status | Awaiting_QC |
| Plaque Notes | A small plaque size, about 3mm in diameter |
| Morphotype | Siphoviridae |
| Number of Genes | 117 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
209 |
| Pitt Freezer Box Grid# |
B5 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
