| Detailed Information for Phage Priscilla |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Laurie Gene Seifert and Eugene Kim |
| Year Found | 2015 |
| Location Found | Nyack, NY USA |
| Finding Institution | Alliance University (formerly Nyack College) |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 41.081081 N, 73.926653 W Map |
| Discovery Notes | Soil was soft, moist black-brown dirt. It also had debris. Soil site had surrounding trees with a lot of roots in the soil. Sample was collected behind Dunbar Hall. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 24, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 375 |
| Genome length (bp) | 58230 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGACGGCGC |
| GC Content | 61.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaque Morphology (after 24hrs):
Diameter: 1-2mm
Clarity of Plaque: Clear
|
| Morphotype | Siphoviridae |
| Number of Genes | 105 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG872841 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.2x10^11 pfu/mL |
| Date of SEA Lysate Titering |
Oct 27, 2015 |
| Pitt Freezer Box# |
22 |
| Pitt Freezer Box Grid# |
G11 |
| Available Files |
| GenBank File for Phamerator | Download |