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Detailed Information for Phage Rita
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Anna Fakhri
Year Found	2021
Location Found	North Easton, MA United States
Finding Institution	Providence College
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	37°C
GPS Coordinates	42.051462 N, 71.134382 W Map
Discovery Notes	A soil sample was collected in North Easton, Massachusetts, on Friday, May 28th, 2021 at 4:26 pm. The soil was slightly damp and approximately 4 inches deep into the ground, and was found underneath a shaded, grassy area next to an apple tree. The weather was cloudy, with 52% humidity and a temperature of 12 °C.
Naming Notes	The phage name "Rita" is named after my aunt Rita who is one of my biggest role models.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Aug 23, 2021
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Approximate Shotgun Coverage	2788
Genome length (bp)	58771
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CGGAAGGCGC
GC Content	61.6%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	F
Subcluster	F1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Blarby (None) MulchSalad (None) Akhila (F1) Alexphander (F1) AlpineSix (F1) ArcusAngelus (F1) Ardmore (F1) Aubs (F1) Awesomesauce (F1) Batiatus (F1) Beakin (F1) BigPhil (F1) Bipolar (F1) Blexus (F1) BlueCrab (F1) BobaPhett (F1) Bobi (F1) BodEinwohner17 (F1) Boomer (F1) Brocalys (F1) Bubbles123 (F1) Burwell21 (F1) BuzzLyseyear (F1) ByChance (F1) Byougenkin (F1) Cabrinians (F1) CaptainTrips (F1) Cerasum (F1) Che8 (F1) Chevrolet (F1) ChickenDinner (F1) Chuckly (F1) Clifton (F1) Coco12 (F1) Cornucopia (F1) Crumble (F1) DaddyRickover (F1) Daenerys (F1) Dante (F1) DaWorst (F1) DeadP (F1) Deb65 (F1) DillTech15 (F1) DirtMcgirt (F1) DLane (F1) DocMcStuffins (F1) Donkeykong (F1) Doomphist (F1) Dorothy (F1) DosHalletts (F1) DotProduct (F1) Doug (F1) Drago (F1) DRBy19 (F1) Eish (F1) EleanorGeorge (F1) Emma (F1) Empress (F1) Enby (F1) Eradicator (F1) Estave1 (F1) Fancypants (F1) Fastidio (F1) Filuzino (F1) Firehouse51 (F1) Flathead (F1) Florinda (F1) Frankie (F1) FreddyB (F1) Fruitloop (F1) Galactic (F1) Gandalph (F1) Geralt (F1) GigiOuiOui (F1) Girafales (F1) Girr (F1) Gorge (F1) GUmbie (F1) Guppsters (F1) Hades (F1) Hamulus (F1) Harley (F1) Hegedechwinu (F1) Hlubikazi (F1) Ibhubesi (F1) IbOuu (F1) IMpilo (F1) Inventum (F1) IrishSherpFalk (F1) JalFarm20 (F1) James (F1) Jarcob (F1) Jinglebell (F1) Job42 (F1) JoeyJr (F1) Juice456 (F1) Juniper1 (F1) Karhdo (F1) Kenuha5 (F1) Kersh (F1) Kimberlium (F1) KingMidas (F1) Kingsley (F1) Koella (F1) Krakatau (F1) KristaRAM (F1) LastJedi (F1) Latretium (F1) Leozinho (F1) Leperchaun (F1) LilMoolah (F1) LittleShirley (F1) Lizziana (F1) Llama (F1) Llij (F1) Lorde (F1) LunaBlu (F1) Madiba (F1) MadMen (F1) Mahavrat (F1) Mandlovu (F1) Mantra (F1) Maravista (F1) Marker (F1) Martik (F1) Mattes (F1) Melissauren88 (F1) MilanaBonita (F1) MilleniumForce (F1) MinionDave (F1) Minnie (F1) Misha28 (F1) MisterCuddles (F1) Modragons (F1) Moonbeam (F1) Mova (F1) Mozy (F1) Ms6 (F1) MulchExplorer (F1) Mutaforma13 (F1) Nimbo (F1) Nitzel (F1) Nivrat (F1) NormanBulbieJr (F1) Ochi17 (F1) OfUltron (F1) Ogopogo (F1) Oksu (F1) OldBen (F1) OlympiaSaint (F1) Ovechkin (F1) OwlsT2W (F1) Pacc40 (F1) Phalconet (F1) Phanphagia (F1) PHappiness (F1) Phasih (F1) Phatniss (F1) PhesterPhotato (F1) Philus (F1) Piper2020 (F1) Pippy (F1) Plumbus (F1) PMC (F1) Poenanya (F1) Polka14 (F1) Pollywog (F1) PopTart (F1) Priscilla (F1) QuickMath (F1) Quico (F1) Radiance (F1) Ramsey (F1) RedBird (F1) Rialto (F1) Rita (F1) RitaG (F1) Rockne (F1) RockyHorror (F1) Royals2015 (F1) Ruby (F1) Saal (F1) Sabbb (F1) Sandalphon (F1) Sarma624 (F1) Sassafras (F1) SassyB (F1) Scottish (F1) Seabastian (F1) Seagreen (F1) SG4 (F1) Shauna1 (F1) ShiLan (F1) ShowerHandel (F1) ShroomBoi (F1) SimranZ1 (F1) SiSi (F1) Sparkdehlily (F1) Spartacus (F1) Spikelee (F1) Spoonbill (F1) StAnnes (F1) Stap (F1) Starcevich (F1) Strokeseat (F1) SuperGrey (F1) SwagPigglett (F1) Taj (F1) TDanisky (F1) TootsiePop (F1) Tootsieroll (F1) Totinger (F1) Tweety (F1) UncleRicky (F1) Velveteen (F1) Veteran (F1) VRedHorse (F1) Wachhund (F1) Wee (F1) Whatsapiecost (F1) Whouxphf (F1) WillSterrel (F1) XFactor (F1) Yorick (F1) Zerg (F1) Zizzle (F1) Avani (F2) Che9d (F2) Demsculpinboyz (F2) Jabbawokkie (F2) Soul22 (F2) Yoshi (F2) Zapner (F2) Squirty (F3) LunaStella (F4) Renaud18 (F4) TChen (F4) ThetaBob (F4) Cornie (F5) Toron (F6)
Annotating Institution	Providence College
Annotation Status	In GenBank
Plaque Notes	The plaques are very small and clear.
Morphotype	Siphoviridae
Number of Genes	104
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Rita_1 (terminase small subunit) Rita_2 (terminase large subunit) Rita_3 (portal protein) Rita_4 (capsid maturation protease) Rita_5 (scaffolding protein) Rita_6 (major capsid protein) Rita_7 (head-to-tail adaptor) Rita_8 (head-to-tail stopper) Rita_9 Rita_10 (tail terminator) Rita_11 (major tail protein) Rita_12 (tail assembly chaperone) Rita_13 (tail assembly chaperone) Rita_14 (tape measure protein) Rita_15 (minor tail protein) Rita_16 (minor tail protein) Rita_17 (minor tail protein) Rita_18 (minor tail protein) Rita_19 (minor tail protein) Rita_20 Rita_21 Rita_22 Rita_23 Rita_24 Rita_25 (helix-turn-helix DNA binding domain protein) Rita_26 Rita_27 Rita_28 Rita_29 Rita_30 Rita_31 (lysin A) Rita_32 (lysin B) Rita_33 (holin) Rita_34 Rita_35 Rita_36 (DnaQ-like DNA polymerase III subunit) Rita_37 Rita_38 Rita_39 Rita_40 Rita_41 Rita_42 Rita_43 Rita_44 (tyrosine integrase) Rita_45 Rita_46 (immunity repressor) Rita_47 (Cro protein) Rita_48 Rita_49 Rita_50 Rita_51 Rita_52 (excise) Rita_53 Rita_54 Rita_55 Rita_56 Rita_57 Rita_58 (WhiB family transcription factor) Rita_59 Rita_60 (WhiB family transcription factor) Rita_61 (helix-turn-helix DNA binding domain protein) Rita_62 Rita_63 Rita_64 Rita_65 (DNA binding protein) Rita_66 (HNH endonuclease) Rita_67 (histidine triad nucleotide binding protein) Rita_68 Rita_69 Rita_70 Rita_71 Rita_72 Rita_73 (DNA helicase) Rita_74 Rita_75 Rita_76 (DNA methylase) Rita_77 Rita_78 Rita_79 Rita_80 Rita_81 (HNH endonuclease) Rita_82 Rita_83 Rita_84 Rita_85 Rita_86 Rita_87 Rita_88 Rita_89 Rita_90 Rita_91 Rita_92 Rita_93 Rita_94 Rita_95 (exonuclease) Rita_96 Rita_97 Rita_98 Rita_99 Rita_100 Rita_101 (glycosyltransferase) Rita_102 Rita_103 (glycosyltransferase) Rita_104 (HNH endonuclease)
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	OP068340
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	136
Pitt Freezer Box Grid#	B6
Available Files
EM Picture	Download
GenBank File for Phamerator	Download

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