| Detailed Information for Phage RitaG |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kevin A. Brighter Jr. |
| Year Found | 2015 |
| Location Found | Bensalem, PA USA |
| Finding Institution | La Salle University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.099400 N, 74.932600 W Map |
| Discovery Notes | Found in dark soil and had a mixture of mulch and flowers planted around it. Retrieved at a depth of 1/2 inch in to soil on a 90 degree Fahrenheit day. |
| Naming Notes | Discovered in my grandmother's garden and her name is Rita. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 13, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1074 |
| Genome length (bp) | 57789 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGACGGCGC |
| GC Content | 61.6% |
| Sequencing Notes | There was one deletion present in 15% of this phage population. Deletion 1 was 5053 bp long, and is between positions 42000 - 47052, inclusive. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Is in Lysogenic cycle. Plaque has a ring around it which makes it seem bigger. the inner part of the the plaque is very clear, while the otter part, or the ring is a little bit cloudy. The full diameter of the the plaque is 1 cm. The diameter of the center section is approximately .6 cm. |
| Morphotype | Siphoviridae |
| Number of Genes | 103 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF668281 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
57.78 |
| Date of SEA Lysate Titering |
Oct 6, 2015 |
| Pitt Freezer Box# |
175 |
| Pitt Freezer Box Grid# |
G5 |
| Available Files |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |